Aspartate Transaminase 抗体
(1 reference)
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- 抗原 See all Aspartate Transaminase (Ast) products
- Aspartate Transaminase (Ast)
- 适用
- Pig
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This Aspartate Transaminase antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 100-601-144
- Supplier
- Rockland
- 原理
- Aspartate Transaminase Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
- 产品特性
- Synonyms: sheep anti-Aspartate Transaminase Antibody, Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
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Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]
Immunogen Type: Native Protein
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anti-Aspartate Transaminase (Ast) antibody (HRP)
Ast 适用: Pig ELISA, WB, DB 宿主: 绵羊 Polyclonal HRP
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- 应用备注
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Application Note: Anti-Aspartate Transaminase has been assayed against 1.0 μg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 96 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Hydrogen sulfide protects the retina from light-induced degeneration by the modulation of Ca2+ influx." in: The Journal of biological chemistry, (2011) (PubMed).
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: "Hydrogen sulfide protects the retina from light-induced degeneration by the modulation of Ca2+ influx." in: The Journal of biological chemistry, (2011) (PubMed).
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- 抗原
- Aspartate Transaminase (Ast)
- 别名
- Aspartate Transaminase (Ast 产品)
- 背景
- Background: Aspartate aminotransferase is involved in the biosynthesis of L-glutamate from L-aspartate or L-cysteine. It is an important regulator of the levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. It acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, Aspartate aminotransferase regulates levels of mercaptopyruvate, an important source of hydrogen sulfide, then mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In eukaryotes there are cytoplasmic, mitochondrial, and chloroplastic isozymes.
- 基因ID
- 396967
- NCBI登录号
- NP_999092
- UniProt
- P00503
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