Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
This antibody detects TLR9. Located in the membrane, and highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver (6).
纯化方法
Purified
免疫原
Peptide corresponding to 15 amino acids near the center of human TLR9
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences (3) and are localized in intracellular acidic compartments such as the phagolysosome (4). Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection (5), TLR9 is strictly dependent on MyD88 (3).Synonyms: Toll-like receptor 9, UNQ5798/PRO19605