beta Amyloid 抗体 (N-Term)
(1 reference)
(2 validations)-
- 抗原 See all beta Amyloid (Abeta) 抗体
- beta Amyloid (Abeta) (Amyloid beta (Abeta))
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抗原表位
- N-Term
-
适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This beta Amyloid antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- This affinity-purified antibody is directed against the amino terminal end of beta amyloid and is useful in determining its presence in various assays. Polyclonal anti-beta amyloid detects human and mouse beta amyloid. Cross reactivity with beta amyloid from other species is likely but has not been determined.
- 产品特性
- Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
- 纯化方法
- affinity purified
- 过滤
- Sterile filtered
- 免疫原
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This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the amino terminus (aa 1-14) of human beta amyloid conjugated to KLH using maleimide.
Immunogen Type: Peptide - 亚型
- IgG
- Top Product
- Discover our top product Abeta Primary Antibody
-
anti-Amyloid beta (Abeta) (C-Term) antibody
Abeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 小鼠 Monoclonal 9E8-1A3 unconjugated
anti-Amyloid beta (Abeta) (AA 711-760) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人 WB, ELISA, IHC, IP, IEM 宿主: 兔 Chimeric 6E10 unconjugated Recombinant Antibody
anti-Amyloid beta (Abeta) (Ser15) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) (pThr743) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal AB9 unconjugated
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- 应用备注
- Affinity purified anti-beta amyloid detects beta amyloid in ELISA, IHC and IF. A 40-50 kD band consistent with a higher MW precursor is detected in western blot using whole tissue extracts from mouse brain. In general, we recommend the use of 4% PFA for paraffin embedded tissues and 10% formalin for frozen tissue for fixation.
- 说明
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Gene Name: APP
- 限制
- 仅限研究用
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- by
- NanoTemper Technologies
- No.
- #104082
- 日期
- 2019.07.23
- 抗原
- ABETA
- Lot Number
- Method validated
- Unfolding Profile
- Positive Control
- ABIN1043866
- Negative Control
- Notes
Passed. ABIN1043866 showed Ti at 83.8°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.
- Primary Antibody
- Secondary Antibody
- Full Protocol
- Load the undiluted sample into Tycho capillary (NanoTemper Technologies, TY-C001).
- Run Tycho measurement.
- Experimental Notes
Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.
生效 #104082 (Unfolding Profile)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Unfolding profile of ABIN1043866. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 83.8 °C.]()
Unfolding profile of ABIN1043866. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 83.8 °C.
Full Methods -
- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104424
- 日期
- 2022.05.12
- 抗原
- beta Amyloid
- Lot Number
- 42581
- Method validated
- Immunohistochemistry
- Positive Control
Adult (24 months) mouse brain
3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde
- Negative Control
We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.
- Notes
The beta Amyloid antibody (Amyloid beta) (N-Term) ABIN95037 works in IHC-P, especially at higher concentrations (1:20 and 1:50), and without the use of any antigen retrieval treatment.
- Primary Antibody
- ABIN95037
- Secondary Antibody
- goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)
- Full Protocol
- Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Wash several times with 0.01 M PBS.
- Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
- After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
- Incubate sections with primary anti-beta amyloid (N-Term) (antibodies Online, ABIN95037, lot 42581) diluted 1:20, 1:50, 1:100, and 1:200 in 0.1 M PBS-BSA-PLL ON at RT.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
- Wash sections 3x 5 min with 0.01M PBS.
- Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
- Experimental Notes
Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min (95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.
生效 #104424 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Staining of beta-amyloid positive cells in the adult mouse brain using ABIN95037 at 40x magnification.]()
Staining of beta-amyloid positive cells in the adult mouse brain using ABIN95037 at 40x magnification.
Full Methods -
- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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-
: "N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies." in: Journal of neuroimmunology, Vol. 88, Issue 1-2, pp. 85-90, (1998) (PubMed).
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: "N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies." in: Journal of neuroimmunology, Vol. 88, Issue 1-2, pp. 85-90, (1998) (PubMed).
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- 抗原
- beta Amyloid (Abeta) (Amyloid beta (Abeta))
- 别名
- Beta Amyloid (Abeta 产品)
- 别名
- AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN-II antibody, PN2 antibody, aaa antibody, abeta antibody, abpp antibody, ad1 antibody, appi antibody, ctfgamma antibody, cvap antibody, pn2 antibody, Abeta antibody, Abpp antibody, Adap antibody, Ag antibody, Cvap antibody, E030013M08Rik antibody, betaApp antibody, amyloid beta precursor protein antibody, amyloid beta (A4) precursor protein antibody, APP antibody, app antibody, App antibody
- 背景
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Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.
Synonyms: Beta amyloid, A-beta - 基因ID
- 351
- NCBI登录号
- NP_000475
- UniProt
- P05067
- 途径
- Inflammasome
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