D Amino Acid Oxidase 抗体
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- 抗原 See all D Amino Acid Oxidase (DAO) 抗体
- D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))
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适用
- Pig
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This D Amino Acid Oxidase antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 (详细)
- Cross reactivity against D-Amino Acid Oxidase from other tissues and species may occur but have not been specifically determined.
- 纯度
- Anti-D-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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D-Amino Acid Oxidase [Pig Kidney]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product DAO Primary Antibody
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- 应用备注
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D-AMINO ACID OXIDASE antibody has been assayed against 1.0 µg of D-Amino Acid Oxidase [Pig Kidney] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20.000 to 1:60.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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