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Alkaline Phosphatase 抗体

Rockland 100-4134 ALP 适用: E. coli WB, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN94942
Supplier Product No.: 100-4134
发货至: 中国
  • 抗原 See all Alkaline Phosphatase (ALP) 抗体
    Alkaline Phosphatase (ALP)
    适用
    • 50
    • 16
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    • 1
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    E. coli
    宿主
    • 50
    • 17
    • 2
    • 1
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    克隆类型
    • 45
    • 26
    多克隆
    标记
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    This Alkaline Phosphatase antibody is un-conjugated
    应用范围
    • 42
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    Western Blotting (WB), ELISA
    Supplier Product No.
    100-4134
    Supplier
    Rockland
    原理
    Alkaline Phosphatase Antibody
    产品特性
    Synonyms: rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody
    纯化方法
    Anti-Alkaline Phosphatase (E.Coli) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.
    免疫原

    Immunogen: Alkaline Phosphatase [E.coli]

    Immunogen Type: Native Protein

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    Discover our top product ALP Primary Antibody
  • 应用备注

    Application Note: Anti-Alkaline Phosphatase (E. Coli) Antibody has been tested against 1.0 μg of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Alkaline Phosphatase (E. Coli) Antibody.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:8,000 - 1:40,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    75 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Epstein, Kiechle, Artiss, Zak: "The clinical use of alkaline phosphatase enzymes." in: Clinics in laboratory medicine, Vol. 6, Issue 3, pp. 491-505, (1986) (PubMed).

    Lange, Millan, Stigbrand, Vessella, Ruoslahti, Fishman: "Placental alkaline phosphatase as a tumor marker for seminoma." in: Cancer research, Vol. 42, Issue 8, pp. 3244-7, (1982) (PubMed).

  • 抗原
    Alkaline Phosphatase (ALP)
    别名
    Alkaline Phosphatase (ALP 产品)
    背景
    Background: Alkaline Phosphatase is an enzyme that is key in the hydrolysis of many different types of molecules, resulting in the removal of phosphate groups from said molecule. Most active in basic environments and a common serum analyte, alkaline phosphatase and its isoenzymes are good indicators of bone, liver, and testicular diseases/cancers in immunoassays and diagnostics. Anti-Alkaline Phosphatase (E. Coli) Antibody is ideal for investigators in Cancer, Immunology, and Molecular Biology.
    基因ID
    945041
    NCBI登录号
    NP_414917
    UniProt
    P00634
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