Calf Intestinal Alkaline Phosphatase (CIAP) 抗体
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- 抗原 See all Calf Intestinal Alkaline Phosphatase (CIAP) products
- Calf Intestinal Alkaline Phosphatase (CIAP)
- 适用
- Cow
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-1135-0100
- Supplier
- Rockland
- 原理
- Alkaline Phosphatase Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
- 产品特性
- Synonyms: goat anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
- 纯化方法
- Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Calf Intestinal Alkaline Phosphatase (CIAP) antibody
CIAP 适用: Cow WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calf Intestinal Alkaline Phosphatase (CIAP) antibody (Biotin)CIAP 适用: Cow WB, ELISA 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Application Note: Anti-Alkaline Phosphatase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None - 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Calf Intestinal Alkaline Phosphatase (CIAP)
- 别名
- Calf Intenstinal Alkaline Phosphatase (CIAP 产品)
- 背景
- Background: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.
- 基因ID
- 280993
- UniProt
- P19111
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