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ALD2 抗体

Rockland 100-4144 适用: Saccharomyces cerevisiae ELISA, IP, WB 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN94894
Supplier Product No.: 100-4144
发货至: 中国
  • 抗原
    ALD2
    适用
    Saccharomyces cerevisiae
    宿主
    • 3
    克隆类型
    • 3
    多克隆
    标记
    • 1
    • 1
    • 1
    非结合性
    应用范围
    ELISA, Immunoprecipitation (IP), Western Blotting (WB)
    Supplier Product No.
    100-4144
    Supplier
    Rockland
    原理
    Aldehyde Dehydrogenase Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Aldehyde Dehydrogenase [Yeast].
    产品特性
    Synonyms: rabbit anti-Aldehyde Dehydrogenase Antibody, Aldehyde dehydrogenase, [NAD(P)+] 1, ALD2, ALD5
    纯化方法
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    免疫原

    Immunogen: Aldehyde Dehydrogenase [Yeast]

    Immunogen Type: Native Protein

  • 应用备注

    Application Note: Anti-Aldehyde Dehydrogenase has been tested by western blot and is suitable to be assayed against 1.0 μg of Aldehyde Dehydrogenase [Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    85 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    ALD2
    背景
    Background: Cytoplasmic aldehyde dehydrogenase is involved in ethanol oxidation. Aldehyde dehydrogenase is required for pantothenic acid production through the conversion of 3-aminopropanal to beta-alanine, an intermediate in pantothenic acid (vitamin B5) and coenzyme A (CoA) biosynthesis.
    基因ID
    855206
    NCBI登录号
    NP_013893
    UniProt
    P47771
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