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ATP1A1 抗体

Rockland 200-9155 ATP1A1 适用: 犬 WB, ELISA, IP 宿主: 小鸡 Polyclonal unconjugated
Rockland
产品编号 ABIN94710
Supplier Product No.: 200-9155
发货至: 中国
  • 抗原 See all ATP1A1 抗体
    ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))
    适用
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    宿主
    • 86
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    小鸡
    克隆类型
    • 86
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    多克隆
    标记
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    This ATP1A1 antibody is un-conjugated
    应用范围
    • 85
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Supplier Product No.
    200-9155
    Supplier
    Rockland
    原理
    ATPase Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].
    产品特性
    Synonyms: chicken anti-ATPase Antibody, Sodium/potassium-transporting ATPase
    纯化方法
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: ATPase, Sodium, Potassium [Canine Kidney]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product ATP1A1 Primary Antibody
  • 应用备注

    Application Note: Anti-ATPase Na+ K+ has been assayed against 1.0 μg of ATPase, Sodium, Potassium in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) code #603-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))
    别名
    ATP1A1 (ATP1A1 产品)
    背景
    Background: Sodium/potassium-transporting ATPase subunit alpha-1 is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
    基因ID
    403992
    NCBI登录号
    NP_001003306
    UniProt
    P50997
    途径
    Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process
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