This product has been assayed by western blot and was found to be reactive against the intact enzyme and enzyme subunits (alpha at 100 kDa and beta at 50 kDa) as well as glycosylated and non-glycosylated enzyme forms. A dilution of 1:100 to 1:500 is suggested for immunoblotting followed by reaction with Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] Rabbit) (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with deionized water (or equivalent)
浓度
10.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
抗原
ATPase (DNAH8)
(Dynein, Axonemal, Heavy Chain 8 (DNAH8))