NAM-DH 抗体 (Biotin)
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- 抗原
- NAM-DH
- 适用
- Pseudomonas
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
- 应用范围
- ELISA, Immunoprecipitation (IP), Western Blotting (WB)
- Supplier Product No.
- 200-106-228-0100
- Supplier
- Rockland
- 原理
- N-Acylmannosamine-1-Dehydrogenase Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified N-Acylmannoseamine-1-Dehydrogenase [Recombinant from Pseudomonas].
- 产品特性
- Synonyms: goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody biotin Conjugation, Biotin Conjugated goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH
- 纯化方法
- Anti-N-Acylmannosamine-1-Dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
Immunogen Type: Recombinant Protein
- 亚型
- IgG
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anti-NAM-DH antibody
适用: Pseudomonas ELISA, IP, WB 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-N-Acylmannosamine-1-Dehydrogenase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of N-Acylmannoseamine-1-Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:35,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:250 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- NAM-DH
- 背景
- Background: Anti-N-Acylmannosamine-1-Dehydrogenase recognizes the protein N-Acylmannosamine 1-Dehydrogenase, a member of the oxidoreductase family that act on CH-OH donors and NAD+ or NADP+ as acceptors. N-acyl-D-mannosamine 1-dehydrogenase catalyzes the reaction in which N-acyl-D-mannosamine and NAD+ are converted to N-acyl-D-mannosaminolactone, NADH, and H+.
- 基因ID
- 7614553
- UniProt
- P22441
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