NAM-DH 抗体
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- 抗原
- NAM-DH
- 适用
- Pseudomonas
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- ELISA, Immunoprecipitation (IP), Western Blotting (WB)
- Supplier Product No.
- 100-101-228
- Supplier
- Rockland
- 原理
- N-Acylmannosamine-1-Dehydrogenase Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified N-Acyl Mannosamine-1-Dehydrogenase [E.
- 产品特性
- Synonyms: goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
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Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
Immunogen Type: Recombinant Protein
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anti-NAM-DH antibody (Biotin)
适用: Pseudomonas ELISA, IP, WB 宿主: 山羊 Polyclonal Biotin
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- 应用备注
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Application Note: Anti-N-Acyl Mannosamine-1-Dehydrogenase has been by western blot and is suitable to be assayed against 1.0 μg of N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 90 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- NAM-DH
- 背景
- Background: N-acylmannosamine 1-dehydrogenase acts on acetyl-D-mannosamine and glycolyl-D-mannosamine. NAM-DH may be applicable to the quantitative determination of sialic acid in serum.
- 基因ID
- 7614553
- UniProt
- P22441
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