Acetylcholinesterase 抗体 (Biotin)
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- 抗原 See all Acetylcholinesterase (AChE) 抗体
- Acetylcholinesterase (AChE)
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适用
- Cow
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Acetylcholinesterase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against Acetyl Cholinesterase from other sources is unknown.
- 纯化方法
- Acetyl Cholinesterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Acetyl Cholinesterase [Bovine Erythrocytes]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product AChE Primary Antibody
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anti-Acetylcholinesterase (AChE) (C-Term) antibody
Verified AChE 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 398-617) antibodyAChE 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (C-Term) antibodyAChE 适用: 人, 大鼠, 小鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 157-332) antibodyAChE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 147-175), (N-Term) antibodyAChE 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB16792 unconjugated
anti-Acetylcholinesterase (AChE) (AA 521-614) antibodyAChE 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 53-162) antibodyAChE 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) antibodyAChE 适用: 人 ELISA, IHC, IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 366-554) antibodyAChE 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Acetylcholinesterase (AChE) (AA 147-175) antibodyAChE 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Anti-Acetyl Cholinesterase Biotin Conjugated Antibody has been assayed against 1.0 µg of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1.000 to 1:2.000 is suggested for this product. This product has been assayed against 1.0 µg of Acetyl Cholinesterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1.000 to 1:2.000 is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Acetylcholinesterase (AChE)
- 别名
- Acetyl Cholinesterase (AChE 产品)
- 别名
- ACEE antibody, ARACHE antibody, N-ACHE antibody, YT antibody, Ache antibody, GB14873 antibody, zgc:92550 antibody, ACE antibody, Dsim\\GD20515 antibody, GD20515 antibody, dsim_GLEANR_4292 antibody, ache antibody, mE1a antibody, mE1b antibody, mE1c antibody, mE1c-long antibody, mE1d antibody, mE1d' antibody, mE1e antibody, arache antibody, n-ache antibody, acetylcholinesterase (Cartwright blood group) antibody, acetylcholinesterase 2 antibody, acetylcholinesterase antibody, Acetylcholine esterase antibody, acetylcholinesterase (Cartwright blood group) L homeolog antibody, collagen type I alpha 2 chain antibody, ACHE antibody, AChE-2 antibody, Ache antibody, ache antibody, Dsim\Ace antibody, ache.L antibody, COL1A2 antibody, ACE-1 antibody
- 背景
- Acetyl Cholinesterase is abundantly expressed in erythrocytes and is part of the type-B carboxylesterase/lipase family. Anti-Acetyl Cholinesterase cancels signal transduction at the neuromuscular junction via hydrolysis of acetylcholine as it is released into the synaptic cleft by the motor neurons. Anti-Acetyl Cholinesterase antibody is ideal for investigators involved in Neuroscience and Neurotransmitter research. Synonyms: Acetylcholinesterase, AChE
- 基因ID
- 540446
- UniProt
- P23795
- 途径
- Skeletal Muscle Fiber Development
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