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Vimentin 抗体

Name: Vimentin 抗体
Brand: antibodies-online
SKU: ABIN94491
Availability: OutOfStock

VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). (Vimentin 抗体)

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-01 ((ABIN94491), diluted 1:400), detected with GAM IgM-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange). (Vimentin 抗体)

IHC (p)

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547. (Vimentin 抗体)

Immunofluorescence staining (rat basophils) (Vimentin 抗体)

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody. (Vimentin 抗体)

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. (Vimentin 抗体)

Separation of HeLa cells stained using anti-Vimentin (VI-01) purified antibody (concentration in sample 5 μg/mL, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining of methanol permeabilisated cells). (Vimentin 抗体)

FACS

Immunocytochemistry analysis of vimentin in methanol/acetone fixed murine 3T3 cells using mouse monoclonal antibody VI-01. (Vimentin 抗体)

ICC

Anti-Vimentin Purified (VI-01) works in WB application under both reducing and non-reducing conditions. (Vimentin 抗体)

9 images

(4 references)

产品编号 ABIN94491

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
适用
342

133

130

60

60

49

42

37

29

21

20

13

13

8

8

6

5

3

3

2

1

1

1

1

1

1

1

1

1
哺乳动物
宿主
195

129

11

6

6

5

2
小鼠
Compatible Secondaries
克隆类型
211

142
单克隆
标记
203

19

14

14

10

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
285

129

106

100

96

63

45

40

39

33

23

19

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

特异性

The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.

交叉反应（详细）

Mammalian

纯化方法

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

纯度

> 95 % (by SDS-PAGE)

免疫原

Pellet of porcine brain cold stable proteins after depolymerization of microtubules.

克隆位点

VI-01

亚型

IgM

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anti-Vimentin (VIM) antibody
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anti-Vimentin (VIM) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20 °C and for 6 min in acetone at -20 °C, (b) fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Sengez, Aygün, Shehwana, Toyran, Tercan Avci, Konu, Stemmler, Alotaibi: "The Transcription Factor Elf3 Is Essential for a Successful Mesenchymal to Epithelial Transition." in: Cells, Vol. 8, Issue 8, (2019) (PubMed).

Kotb, Hierholzer, Kemler: "Replacement of E-cadherin by N-cadherin in the mammary gland leads to fibrocystic changes and tumor formation." in: Breast cancer research : BCR, Vol. 13, Issue 5, pp. R104, (2012) (PubMed).

Bacáková, Mares, Lisá, Svorcík: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." in: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, (2000) (PubMed).

Dráberová, Dráber, Havlícek, Viklický: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, (1989) (PubMed).
抗原

Vimentin (VIM)

别名

Vimentin (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

背景

Vimentin,Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.

基因ID

7431

UniProt

P08670

途径

Caspase Cascade in Apoptosis