Transferrin 抗体
(3 references)-
- 抗原 See all Transferrin (TF) 抗体
- Transferrin (TF)
-
适用
- 人, Pig, 兔
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Transferrin antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
- 特异性
- The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
- 无交叉反应
- Cow, 犬, 马, 绵羊
- 交叉反应 （详细）
- Human, Rabbit, Porcine
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Purified porcine transferrin.
- 克隆位点
- HTF-14
- 亚型
- IgG1
- Top Product
- Discover our top product TF Primary Antibody
-
anti-Transferrin (TF) (AA 361-683) antibody
TF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 22-176) antibodyTF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 551-650) antibodyTF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 20-49), (N-Term) antibodyTF 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Transferrin (TF) (AA 284-575) antibodyTF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 101-135) antibodyTF 适用: 人 WB, IF 宿主: 兔 Polyclonal RB55977 unconjugated
-
- 应用备注
-
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: placenta.
Western blotting: non-reducing conditions, recommended dilution: 1-2 μg/mL. - 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
-
-
: "Maghemite nanoparticles stabilize the protein corona formed with transferrin presenting different iron-saturation levels." in: Nanoscale, (2019) (PubMed).
: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).
: "Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding." in: British journal of haematology, Vol. 59, Issue 3, pp. 435-41, (1985) (PubMed).
-
: "Maghemite nanoparticles stabilize the protein corona formed with transferrin presenting different iron-saturation levels." in: Nanoscale, (2019) (PubMed).
-
- 抗原
- Transferrin (TF)
- 别名
- Transferrin (TF 产品)
- 背景
- Transferrin,Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.,TFQTL1, PRO1557, PRO2086
- 基因ID
- 7018
- UniProt
- P02787
- 途径
- Transition Metal Ion Homeostasis
-
