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SYK 抗体 (AA 5-360)

Name: SYK 抗体 (AA 5-360)
Brand: antibodies-online
SKU: ABIN94475
Availability: OutOfStock

SYK 适用: 人, 小鼠, 大鼠 WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal SYK-01 unconjugated

Western blotting analysis of human Syk using mouse monoclonal antibody SYK-01 on lysates of Ramos and Jurkat (negative control) cells under reducing and non-reducing conditions. (SYK 抗体 (AA 5-360))

Immunofluorescence staining of Syk in human primary fibroblasts using anti-Syk (; green). (SYK 抗体 (AA 5-360))

Western Blotting analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). (SYK 抗体 (AA 5-360))

Immunofluorescence staining of Syk in human HeLa cell line using anti-Syk (SYK 抗体 (AA 5-360))

Immunocytochemistry analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01, green). (SYK 抗体 (AA 5-360))

ICC

Immunocytochemistry analysis of Syk in human HeLa cell line using anti-Syk (SYK-01, green). (SYK 抗体 (AA 5-360))

ICC

6 images

(4 references)

产品编号 ABIN94475

发货至: 中国

Datasheet as PDF

抗原 See all SYK 抗体

SYK (Spleen tyrosine Kinase (SYK))
抗原表位
34

29

19

13

11

10

8

8

8

7

6

6

5

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 5-360
适用
181

88

58

11

5

4

4

3

3

1

1
人, 小鼠, 大鼠
宿主
171

13

3
小鼠
Compatible Secondaries
克隆类型
174

12
单克隆
标记
101

12

10

8

5

5

4

4

4

4

4

4

2

2

2

2

2

2

2

2

2

2

2
This SYK antibody is un-conjugated
应用范围
147

86

48

26

21

19

14

14

11

4

4

3

2

2

2

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

特异性

The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk, an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).

交叉反应（详细）

Mouse, Human, Rat, Other not tested

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Recombinant fragment (aa 5-360) of human Syk.

克隆位点

SYK-01

亚型

IgG1
anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibody
SYK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr525) antibody
SYK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr348) antibody
SYK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (Internal Region) antibody
SYK 适用: 人, 小鼠, 大鼠 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (AA 217-356) antibody
SYK 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2G11E6 unconjugated

anti-Spleen tyrosine Kinase (SYK) (AA 387-417), (C-Term) antibody
SYK 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03097 unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr525), (pTyr526) antibody
SYK 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibody
SYK 适用: 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibody
SYK 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (AA 1-635) antibody
SYK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

应用备注

Western blotting: Recommended dilution: 1-2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: tonsil B cells.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Hasan, Rahman, Sebo, Osicka: "Distinct Spatiotemporal Distribution of Bacterial Toxin-Produced Cellular cAMP Differentially Inhibits Opsonophagocytic Signaling." in: Toxins, Vol. 11, Issue 6, (2020) (PubMed).

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, (2006) (PubMed).

Hálová, Dráberová, Dráber: "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor." in: International immunology, Vol. 14, Issue 2, pp. 213-23, (2002) (PubMed).

Arudchandran, Brown, Peirce, Song, Zhang, Siraganian, Blank, Rivera: "The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH(2)-terminal kinase 1." in: The Journal of experimental medicine, Vol. 191, Issue 1, pp. 47-60, (2000) (PubMed).
抗原

SYK (Spleen tyrosine Kinase (SYK))

别名

Syk (SYK 产品)

别名

p72-Syk antibody, Sykb antibody, p72syk antibody, spleen associated tyrosine kinase antibody, spleen tyrosine kinase antibody, syk antibody, SYK antibody, Syk antibody

背景

Spleen associated tyrosine kinase,Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.,p72Syk

基因ID

6850

UniProt

P43405

途径

Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling