p53 抗体
(4 references)-
- 抗原 See all p53 (TP53) 抗体
- p53 (TP53) (Tumor Protein P53 (TP53))
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适用
- 人, Non-Human Primate
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This p53 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 特异性
- The antibody BP53-12 recognizes a defined epitope (aa 16-25) on human p53, a 50 kDa intracellular tumour suppressor found in increased amounts in a wide variety of transformed cells, it is frequently mutated or inactivated in many types of cancer.
- 交叉反应 （详细）
- Human, Non-Human Primates
- 纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Bacterially expressed full-length wild-type p53
- 克隆位点
- BP53-12
- 亚型
- IgG2a
- Top Product
- Discover our top product TP53 Primary Antibody
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anti-Tumor Protein P53 (TP53) (pSer392) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 1-100) antibodyTP53 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer33) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (acLys319) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer20) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blotting: Recommended dilution: 1-2 μg/mL, overnight at 4 °C, positive control: RAMOS human lymphoma cell line, non-reducing conditions. SDS-PAGE (12 % separating gel).
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Creation of a novel telomere-cutting endonuclease based on the EN domain of telomere-specific non-long terminal repeat retrotransposon, TRAS1." in: Mobile DNA, Vol. 1, Issue 1, pp. 13, (2010) (PubMed).
: "Epitope analysis of the human p53 tumour suppressor protein." in: Folia biologica, Vol. 43, Issue 1, pp. 49-51, (1997) (PubMed).
: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, (1991) (PubMed).
: "p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 2, pp. 196-202, (1991) (PubMed).
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: "Creation of a novel telomere-cutting endonuclease based on the EN domain of telomere-specific non-long terminal repeat retrotransposon, TRAS1." in: Mobile DNA, Vol. 1, Issue 1, pp. 13, (2010) (PubMed).
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- 抗原
- p53 (TP53) (Tumor Protein P53 (TP53))
- 别名
- p53 (TP53 产品)
- 背景
- Tumor protein p53,The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.,BCC7, TRP53, TP53, LFS1
- 基因ID
- 7157
- UniProt
- P04637
- 途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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