HLAG 抗体 (Biotin)
(6 references)-
- 抗原 See all HLAG 抗体
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HLAG antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- The antibody MEM-G/1 reacts with an extracellular epitope of denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
- 免疫原
- Denatured bacterially expressed recombinant human HLA-G heavy chain.
- 克隆位点
- MEM-G-1
- 亚型
- IgG1
- Top Product
- Discover our top product HLAG Primary Antibody
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anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody
HLAG 适用: 人 WB, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-G-1 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 PE
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibodyHLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibodyHLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal 87G FITC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 FITC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)HLAG 适用: 人 ELISA, FACS, IHC (fro), IP, ICC 宿主: 小鼠 Monoclonal MEM-G-9 Biotin
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (APC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 APC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal 87G PE
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 62-89) antibodyHLAG 适用: 人 WB, ELISA, FACS, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Expression of HLA-G by mast cells is associated with hepatitis C virus-induced liver fibrosis." in: Journal of hepatology, Vol. 60, Issue 2, pp. 245-52, (2014) (PubMed).
: "p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations." in: The American journal of surgical pathology, Vol. 28, Issue 9, pp. 1177-83, (2004) (PubMed).
: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, (2002) (PubMed).
: "Uveal melanoma: no expression of HLA-G." in: Investigative ophthalmology & visual science, Vol. 42, Issue 13, pp. 3081-4, (2001) (PubMed).
: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, (2000) (PubMed).
: "Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment." in: Tissue antigens, Vol. 56, Issue 1, pp. 30-7, (2000) (PubMed).
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: "Expression of HLA-G by mast cells is associated with hepatitis C virus-induced liver fibrosis." in: Journal of hepatology, Vol. 60, Issue 2, pp. 245-52, (2014) (PubMed).
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- 抗原
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
- 别名
- HLA-G (HLAG 产品)
- 别名
- MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody
- 背景
- Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
- 基因ID
- 3135
- UniProt
- P17693
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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