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TUBG1 抗体 (C-Term)

Name: TUBG1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN94313
Availability: OutOfStock

TUBG1 适用: 人, 大鼠, 小鼠, Cow, 小鸡, 植物, Protozoa, Pig WB, ICC, FACS 宿主: 小鼠 Monoclonal TU-30 unconjugated

Western blotting analysis of human gamma-tubulin using mouse monoclonal antibody TU-30 on lysates of various cell lines under reducing and non-reducing conditions. (TUBG1 抗体 (C-Term))

Separation of MCF-7 cells stained using anti-gamma-Tubulin (TU-30) purified antibody (concentration in sample 9 μg/mL, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining). (TUBG1 抗体 (C-Term))

FACS

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (; direct conjugate with Dyomics 547, red). (TUBG1 抗体 (C-Term))

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). (TUBG1 抗体 (C-Term))

Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). (TUBG1 抗体 (C-Term))

ICC

Immunocytochemistry staining of murine fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red). (TUBG1 抗体 (C-Term))

ICC

Immunocytochemistry staining of microtubular networks in 3T3 mouse fibroblasts. (TUBG1 抗体 (C-Term))

ICC

8 images

(8 references)

产品编号 ABIN94313

发货至: 中国

Datasheet as PDF

抗原 See all TUBG1 抗体

TUBG1 (Tubulin, gamma 1 (TUBG1))
抗原表位
13

8

7

5

5

3

2

2

2

1

1

1

1
C-Term
适用
60

29

25

5

5

5

5

4

4

3

3

2

2

1

1

1

1
人, 大鼠, 小鼠, Cow, 小鸡, 植物, Protozoa, Pig
宿主
46

17
小鼠
Compatible Secondaries
克隆类型
44

19
单克隆
标记
38

4

4

3

2

2

2

2

1

1

1

1

1

1
This TUBG1 antibody is un-conjugated
应用范围
45

22

10

10

9

7

5

2

1

1

1

1
Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

特异性

The antibody TU-30 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.

交叉反应（详细）

Human, Porcine, Mouse, Rat, Bovine, Chicken, Protozoa, Plants

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

C-terminal peptide of gamma-tubulin counjugated to KLH.

克隆位点

TU-30

亚型

IgG1
anti-Tubulin, gamma 1 (TUBG1) antibody
TUBG1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Tubulin, gamma 1 (TUBG1) (AA 434-449) antibody
TUBG1 适用: 植物, Protozoa WB, ICC 宿主: 小鼠 Monoclonal TU-32 unconjugated

anti-Tubulin, gamma 1 (TUBG1) (AA 29-184) antibody
TUBG1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, gamma 1 (TUBG1) antibody
TUBG1 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 3E4 unconjugated

anti-Tubulin, gamma 1 (TUBG1) (AA 1-451) antibody
TUBG1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, gamma 1 (TUBG1) (N-Term) antibody
TUBG1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, gamma 1 (TUBG1) antibody
TUBG1 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

anti-Tubulin, gamma 1 (TUBG1) (AA 434-449), (C-Term) antibody
TUBG1 适用: 人, 大鼠, 小鼠, 小鸡, 植物, Protozoa, Pig WB, IF 宿主: 小鼠 Monoclonal TU-30 unconjugated

anti-Tubulin, gamma 1 (TUBG1) (C-Term) antibody
TUBG1 适用: 人, 非洲爪蟾, Rodents WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, gamma 1 (TUBG1) antibody
TUBG1 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Immunocytochemistry: Recommended dilution: 1-2 μg/mL. Staining technique: (a) Fix cells for 10 min in methanol at -20 °C and for 6 min in acetone at -20 °C, (b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.
Western blotting: Recommended dilution 1-2 μg/mL, reducing conditions.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Vulprecht, David, Tibelius, Castiel, Konotop, Liu, Bestvater, Raab, Zentgraf, Izraeli, Krämer: "STIL is required for centriole duplication in human cells." in: Journal of cell science, (2012) (PubMed).

Löffler, Fechter, Matuszewska, Saffrich, Mistrik, Marhold, Hornung, Westermann, Bartek, Krämer: "Cep63 recruits Cdk1 to the centrosome: implications for regulation of mitotic entry, centrosome amplification, and genome maintenance." in: Cancer research, Vol. 71, Issue 6, pp. 2129-39, (2011) (PubMed).

Koledova, Kafkova, Kraemer, Divoky: "DNA damage-induced degradation of Cdc25A does not lead to inhibition of Cdk2 activity in mouse embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 3, pp. 450-61, (2010) (PubMed).

Katsetos, Reddy, Dráberová, Smejkalová, Del Valle, Ashraf, Tadevosyan, Yelin, Maraziotis, Mishra, Mörk, Legido, Nissanov, Baas, de Chadarévian, Dráber: "Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines." in: Journal of neuropathology and experimental neurology, Vol. 65, Issue 5, pp. 465-77, (2006) (PubMed).

Sulimenko, Dráberová, Sulimenko, Macurek, Richterová, Dráber, Dráber: "Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7243-53, (2006) (PubMed).

Libusová, Sulimenko, Sulimenko, Hozák, Dráber: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, (2004) (PubMed).

Binarová, Cenklová, Hause, Kubátová, Lysák, Dolezel, Bögre, Dráber: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, (2000) (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).
抗原

TUBG1 (Tubulin, gamma 1 (TUBG1))

别名

gamma-tubulin 1 (TUBG1 产品)

别名

GCP-1 antibody, TUBG antibody, TUBGCP1 antibody, TUBC antibody, TUBG3 antibody, gamma-tubulin antibody, Tubg2 antibody, xgam antibody, 1500010O08Rik antibody, AI451582 antibody, AI503389 antibody, Tubg antibody, tubgl antibody, zgc:55894 antibody, tubulin gamma 1 antibody, gamma-tubulin 1 antibody, gamma-tubulin antibody, tubulin gamma 1 L homeolog antibody, Tubulin gamma chain antibody, tubulin, gamma 1 antibody, tubulin gamma-1 chain antibody, TUBG1 antibody, tubg1 antibody, tubg1.L antibody, tbg-1 antibody, Tubg1 antibody, LOC101686824 antibody, LOC101103906 antibody

背景

Tubulin gamma 1,The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.,TUBG

基因ID

7283

UniProt

P23258

途径

Microtubule Dynamics, M Phase