Keratin 7/17 抗体
(1 reference)-
- 抗原 See all Keratin 7/17 (KRT7/17) 抗体
- Keratin 7/17 (KRT7/17)
- 适用
- 人, Cow, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 7/17 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
- 无交叉反应
- 小鼠, 兔, 大鼠
- 交叉反应 （详细）
- Porcine, Human, Bovine
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
- 克隆位点
- C-46
- 亚型
- IgG1
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anti-Keratin 7/17 (KRT7/17) antibody
KRT7/17 适用: 人, Cow WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow, Pig WB, IHC, IP, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, pretreatment: 0,1 % pepsin, 30 min, 37 °C, incubation 4 °C overnight, chromogen DAB, positive tissue: pancreas.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: HeLa cells. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).
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: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).
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- 抗原
- Keratin 7/17 (KRT7/17)
- 别名
- Cytokeratin 7+17 (KRT7/17 产品)
- 别名
- KRT7 antibody, CK7 antibody, keratin 7 antibody, KRT7 antibody
- 背景
- Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K7, K17, CK7, CD17, CK17, CYK7, CYK17, KRT7, KRT17, SCL, K2C7
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