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pan Keratin 抗体

Name: pan Keratin 抗体
Brand: antibodies-online
SKU: ABIN94275
Availability: OutOfStock

panKRT 适用: 哺乳动物 FACS, WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-11 unconjugated

Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (pan Keratin 抗体)

IHC

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC). (pan Keratin 抗体)

FACS

Immunocytochemistry staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 ((ABIN94275), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green). (pan Keratin 抗体)

ICC

Immunohistochemistry staining of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11). (pan Keratin 抗体)

IHC (p)

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC). (pan Keratin 抗体)

FACS

Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions. (pan Keratin 抗体)

6 images

(6 references)

产品编号 ABIN94275

发货至: 中国

Datasheet as PDF

抗原 See all pan Keratin (panKRT) 抗体

pan Keratin (panKRT)
适用
84

61

40

35

22

21

20

20

19

16

16

15

10

4

2

1

1

1
哺乳动物
宿主
53

34

3
小鼠
Compatible Secondaries
克隆类型
56

34
单克隆
标记
34

6

4

4

4

4

4

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This pan Keratin antibody is un-conjugated
应用范围
56

54

50

24

19

16

14

14

8

6

6

5

3

2
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

特异性

The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

交叉反应（详细）

Mammalian

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

克隆位点

C-11

亚型

IgG1
anti-pan Keratin (panKRT) antibody
panKRT 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-pan Keratin (panKRT) antibody (FITC)
panKRT 适用: 哺乳动物 FACS 宿主: 小鼠 Monoclonal C-11 FITC

anti-pan Keratin (panKRT) antibody
panKRT 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7H8C4 unconjugated

anti-pan Keratin (panKRT) (AA 331-430) antibody
panKRT 适用: 人, 大鼠 IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-pan Keratin (panKRT) (pan) antibody
panKRT 适用: 人, 大鼠, 小鼠 WB, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated

anti-pan Keratin (panKRT) antibody (PE)
panKRT 适用: 哺乳动物 FACS 宿主: 小鼠 Monoclonal C-11 PE

anti-pan Keratin (panKRT) antibody (APC)
panKRT 适用: 哺乳动物 FACS 宿主: 小鼠 Monoclonal C-11 APC

anti-pan Keratin (panKRT) antibody (Biotin)
panKRT 适用: 哺乳动物 FACS, WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-11 Biotin

anti-pan Keratin (panKRT) antibody
panKRT 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-000R unconjugated Recombinant Antibody

anti-pan Keratin (panKRT) antibody
panKRT 适用: 人, 大鼠, Pig, 豚鼠, 犬, 兔, 小鸡, Hamster, Quail FACS, WB, IHC, ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK103 unconjugated

应用备注

Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 2-8 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Haun, Devaraj, Marinelli, Lee, Weissleder: "Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry." in: ACS nano, Vol. 5, Issue 4, pp. 3204-13, (2011) (PubMed).

Bianchi, Bombelli, Raimondo, Torsello, Angeloni, Ferrero, Di Stefano, Chinello, Cifola, Invernizzi, Brambilla, Magni, Pitto, Zanetti, Mocarelli, Perego: "Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1660-70, (2010) (PubMed).

Broekema, Harmsen, Koerts, Petersen, van Luyn, Navis, Popa: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).

Hamakawa, Sumida, Tanioka, Sogawa, Yamada: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
抗原

pan Keratin (panKRT)

别名

Cytokeratin (Pan-reactive) (panKRT 产品)

别名

xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody

背景

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT