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CD8 抗体 (PE)

Name: CD8 抗体 (PE)
Brand: antibodies-online
SKU: ABIN94235
Availability: OutOfStock

CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD8 (MEM-31) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD8 抗体 (PE))

FACS

Surface staining of human peripheral blood lymphocytes using anti-human CD8 (CD8 抗体 (PE))

FACS

Separation of human CD8 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD8 (MEM-31) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD8 抗体 (PE))

FACS

3 images

(4 references)

产品编号 ABIN94235

发货至: 中国

Datasheet as PDF

抗原 See all CD8 抗体

CD8
适用
191

66

25

12

12

7

7

6

6

6

5

5

5

3

3

3

2

2

2

1
人
宿主
215

48

36
小鼠
Compatible Secondaries
克隆类型
261

30

2
单克隆
标记
96

43

33

26

13

7

5

5

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 antibody is conjugated to PE
应用范围
261

64

54

52

49

43

30

21

17

14

14

14

8

5

4

3

2

1

1

1

1

1
Flow Cytometry (FACS)

特异性

The antibody MEM-31 recognizes a conformationally-dependent extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa). The antibody does not react with formaldehyde-fixed cells, negative in Western blotting application.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Crude thymus membrane fraction.

克隆位点

MEM-31

亚型

IgG2a

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CD8 适用: 人 IHC, IP, WB, ELISA 宿主: 兔 Monoclonal 3C3 unconjugated Recombinant Antibody

anti-CD8 (CD8) antibody
CD8 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-31 unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 适用: Cat FACS 宿主: 小鼠 Monoclonal fCD8 PE

anti-CD8 (CD8) antibody (Biotin)
CD8 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-31 Biotin

anti-CD8 (CD8) antibody (FITC)
CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 FITC

anti-CD8 (CD8) antibody (PerCP)
CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PerCP

anti-CD8 (CD8) antibody (APC)
CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 APC

anti-CD8 (CD8) (AA 135-235) antibody
CD8 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-008R unconjugated Recombinant Antibody

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Linnebacher, Wienck, Boeck, Klar: "Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO)." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 841451, (2010) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Estefanía, Flores, Gómez-Lozano, Aguilar, López-Botet, Vilches: "Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4402-10, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).
抗原

CD8

别名

CD8 (CD8 产品)

别名

CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

背景

The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,p32, LEU2