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Transferrin Receptor 抗体 (Biotin)

Name: Transferrin Receptor 抗体 (Biotin)
Brand: antibodies-online
SKU: ABIN94222
Availability: OutOfStock

TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 Biotin

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC). (Transferrin Receptor 抗体 (Biotin))

FACS

Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE . (Transferrin Receptor 抗体 (Biotin))

FACS

Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (; green). (Transferrin Receptor 抗体 (Biotin))

Separation of cells stained using anti-human CD71 (MEM-75) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC, red-filled) from cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (surface staining). (Transferrin Receptor 抗体 (Biotin))

FACS

4 images

(1 reference)

产品编号 ABIN94222

发货至: 中国

Datasheet as PDF

抗原 See all Transferrin Receptor (TFRC) 抗体

Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
适用
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人
宿主
112

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小鼠
Compatible Secondaries
克隆类型
143

100

1
单克隆
标记
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This Transferrin Receptor antibody is conjugated to Biotin
应用范围
139

134

72

71

49

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Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

特异性

The antibody MEM-75 reacts with an extracellular epitope of CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors, it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

免疫原

NALM-6 human pre-B cell line

克隆位点

MEM-75

亚型

IgG1

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anti-Transferrin Receptor (p90, CD71) (TFRC) antibody
TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 578-765) antibody
TFRC 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) antibody
TFRC 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 1-140) antibody
TFRC 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) antibody
TFRC 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 608-727) antibody
TFRC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A1B2 unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 621-720) antibody
TFRC 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 567-744) antibody
TFRC 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 572-757) antibody
TFRC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 567-744) antibody
TFRC 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated

应用备注

Flow cytometry: Recommended dilution: 2-5 μg/mL.

说明

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Beck, Balogh, Kis, Izsépi, Cervenak, László, Bíró, Liliom, Mocsár, Vámosi, Füst, Matko: "New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production." in: Journal of lipid research, Vol. 51, Issue 2, pp. 286-96, (2010) (PubMed).
抗原

Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

别名

CD71 (TFRC 产品)

别名

Trfr antibody, LOC100219280 antibody, tfr antibody, cd71 antibody, tfr1 antibody, trfr antibody, CD71 antibody, T9 antibody, TFR antibody, TFR1 antibody, TR antibody, TRFR antibody, p90 antibody, 2610028K12Rik antibody, AI195355 antibody, AI426448 antibody, AU015758 antibody, E430033M20Rik antibody, Mtvr-1 antibody, Mtvr1 antibody, transferrin receptor antibody, TFRC antibody, Tfrc antibody, tfrc antibody

背景

Transferrin receptor,CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH . CD71 is also involved in uptake of non-transferrin bound iron.,TfR1, TfR, TR, Trfr, T9, p90, TFRC

基因ID

7037

UniProt

P02786

途径

Transition Metal Ion Homeostasis