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ICAM1 抗体 (PE)

Name: ICAM1 抗体 (PE)
Brand: antibodies-online
SKU: ABIN94181
Availability: OutOfStock

ICAM1 适用: 人 FACS 宿主: 小鼠 Monoclonal 1H4 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD54 (1H4) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (ICAM1 抗体 (PE))

FACS

Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. (ICAM1 抗体 (PE))

FACS

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD54 (1H4) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (ICAM1 抗体 (PE))

FACS

3 images

(1 reference)

产品编号 ABIN94181

发货至: 中国

Datasheet as PDF

抗原 See all ICAM1 抗体

ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
适用
215

73

41

11

5

5

4

3

1

1
人
宿主
123

115

22

3

1

1

1
小鼠
Compatible Secondaries
克隆类型
140

123

1
单克隆
标记
121

26

21

17

11

5

4

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This ICAM1 antibody is conjugated to PE
应用范围
164

149

63

57

48

40

34

32

28

28

24

21

12

10

6

4

3

2

2

1

1

1

1
Flow Cytometry (FACS)

特异性

The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells, the expression of CD54 is upregulated by activation.

交叉反应（详细）

Other not tested, Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Raji cells and spleen cells fused with NS1 cells

克隆位点

1H4

亚型

IgG2b

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anti-Intercellular Adhesion Molecule 1 (ICAM1) antibody
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anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 102-306) antibody
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anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 41-464) antibody
ICAM1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated

anti-Intercellular Adhesion Molecule 1 (ICAM1) (pTyr512) antibody
ICAM1 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 41-464) antibody
ICAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 27-209) antibody
ICAM1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 1-532) antibody
ICAM1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3H8-2G6 unconjugated

anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 313-327) antibody
Verified ICAM1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Williams, Chaudhry, Goodfellow, Lea, Evans: "Interactions of decay-accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites." in: The Journal of general virology, Vol. 85, Issue Pt 3, pp. 731-8, (2004) (PubMed).
抗原

ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

别名

CD54 (ICAM1 产品)

别名

BB2 antibody, CD54 antibody, P3.58 antibody, Icam-1 antibody, Ly-47 antibody, MALA-2 antibody, ICAM antibody, ICAM-1 antibody, ICAM1 antibody, intercellular adhesion molecule 1 antibody, intercellular adhesion molecule-1 antibody, ICAM1 antibody, Icam1 antibody, ICAM-1 antibody

物质类

Viral Protein

背景

Intercellular adhesion molecule 1,CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.,ICAM-1, BB2, P3.58

基因ID

3383

UniProt

P05362

途径

Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling