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ICAM1 抗体

ICAM1 适用: 人, 大鼠, Cow WB, FACS, ELISA, IHC (p), ICC 宿主: 小鼠 Monoclonal MEM-111 unconjugated

Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. (ICAM1 抗体)

Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111. (ICAM1 抗体)

Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE. (ICAM1 抗体)

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0. (ICAM1 抗体)

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0. (ICAM1 抗体)

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0. (ICAM1 抗体)

6 images

(7 references)

产品编号 ABIN94177

发货至: 中国

Datasheet as PDF

抗原 See all ICAM1 抗体

ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
适用
210

74

38

7

5

4

3

3

1

1
人, 大鼠, Cow
宿主
122

109

23

3

1

1

1
小鼠
Compatible Secondaries
克隆类型
133

124

1
单克隆
标记
116

24

22

17

11

5

4

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This ICAM1 antibody is un-conjugated
应用范围
161

144

61

58

43

40

32

29

28

27

22

19

12

10

6

4

4

3

2

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

特异性

The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation, it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

交叉反应（详细）

Rat, Human, Bovine

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Raji human Burkitt's lymphoma cell line

克隆位点

MEM-111

亚型

IgG2a

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anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 41-464) antibody
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anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 313-327) antibody
Verified ICAM1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 250-480) antibody
ICAM1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Flow cytometry: Recommended dilution: 0.5-4 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: thymus, RE cells.
Western blotting: Positive control: RAJI cell line K562 leukemia cell line, JY cell line, aa ctivated T-lymphocytes, non-reducing conditions.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Lee, Sieling, Ochoa, Krutzik, Guo, Hernandez, Rea, Cheng, Colonna, Modlin: "LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8128-36, (2007) (PubMed).

Burdick, McCaffery, Kim, Bochner, Konstantopoulos: "Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow." in: American journal of physiology. Cell physiology, Vol. 284, Issue 4, pp. C977-87, (2003) (PubMed).

Hertel, Lacaille, Strobl, Mellins, Mocarski: "Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus." in: Journal of virology, Vol. 77, Issue 13, pp. 7563-74, (2003) (PubMed).

Tachimoto, Kikuchi, Hudson, Bickel, Hamilton, Bochner: "Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases." in: American journal of respiratory cell and molecular biology, Vol. 26, Issue 6, pp. 645-9, (2002) (PubMed).

Bacáková, Mares, Lisá, Svorcík: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." in: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, (2000) (PubMed).

Nagaraju, Raben, Merritt, Loeffler, Kirk, Plotz: "A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli." in: Clinical and experimental immunology, Vol. 113, Issue 3, pp. 407-14, (1998) (PubMed).

Leeuwenberg, Smeets, Neefjes, Shaffer, Cinek, Jeunhomme, Ahern, Buurman: "E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro." in: Immunology, Vol. 77, Issue 4, pp. 543-9, (1993) (PubMed).
抗原

ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

别名

CD54 (ICAM1 产品)

别名

BB2 antibody, CD54 antibody, P3.58 antibody, Icam-1 antibody, Ly-47 antibody, MALA-2 antibody, ICAM antibody, ICAM-1 antibody, ICAM1 antibody, intercellular adhesion molecule 1 antibody, intercellular adhesion molecule-1 antibody, ICAM1 antibody, Icam1 antibody, ICAM-1 antibody

物质类

Viral Protein

背景

Intercellular adhesion molecule 1,CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.,ICAM-1, BB2, P3.58

基因ID

3383

UniProt

P05362

途径

Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling

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