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CD5 抗体

Name: CD5 抗体
Brand: antibodies-online
SKU: ABIN94161
Availability: OutOfStock

CD5 适用: 人 FACS, WB, IP, ELISA, IHC (p) 宿主: 小鼠 Monoclonal MEM-32 unconjugated

Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (MEM-32) purified antibody (concentration in sample 3 μg/mL, GAM APC). (CD5 抗体)

FACS

Western blotting analysis of human CD5 using mouse monoclonal antibodies MEM-32, CRIS1, and L17F12 on laurylmaltoside lysates of Jurkat cells and of K562 cells (negative control) under non-reducing conditions. (CD5 抗体)

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (MEM-32) purified antibody (concentration in sample 3 μg/mL, GAM APC). (CD5 抗体)

FACS

3 images

(1 reference)

产品编号 ABIN94161

发货至: 中国

Datasheet as PDF

抗原 See all CD5 抗体

CD5
适用
229

80

53

26

22

16

15

12

11

11

11

9

9

2

1
人
宿主
168

82

50

1
小鼠
Compatible Secondaries
克隆类型
225

75
单克隆
标记
101

36

31

27

17

8

7

5

5

4

4

4

4

4

4

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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1
This CD5 antibody is un-conjugated
应用范围
229

103

70

65

61

49

30

24

18

17

13

13

12

6

3

3

2

2

2

2

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

特异性

The antibody MEM-32 reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes).

交叉反应（详细）

Human

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Crude thymus membrane fraction.

克隆位点

MEM-32

亚型

IgG1

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anti-CD5 (CD5) antibody
CD5 适用: 人 FACS, WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal L17F12 unconjugated

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CD5 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6C3B2 unconjugated

anti-CD5 (CD5) (AA 403-495) antibody
CD5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F7 unconjugated

anti-CD5 (CD5) antibody
CD5 适用: 人 FACS, WB, IP, ELISA, IHC (fro) 宿主: 小鼠 Monoclonal CRIS1 unconjugated

anti-CD5 (CD5) antibody (PE)
CD5 适用: 人 FACS 宿主: 小鼠 Monoclonal L17F12 PE

应用备注

ELISA: The antibody MEM-32 can be used in the Sandwich ELISA as the capture antibody in pair with the detection antibody CRIS1.
Immunohistochemistry (paraffin sections): Recommended dilution: 20 μg/mL, positive tissue: spleen.
Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Laurylmaltoside lysing buffer, non-reducing conditions, recommended dilution: 1-2 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).
抗原

CD5

别名

CD5 (CD5 产品)

别名

CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody

背景

CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1

基因ID

921

UniProt

P06127