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CD48 抗体 (FITC)

Name: CD48 抗体 (FITC)
Brand: antibodies-online
SKU: ABIN94159
Availability: OutOfStock

CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 FITC

Separation of human CD48 positive lymphocytes (red-filled) from human CD48 negative neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD48 (MEM-102) FITC (20 μL reagent / 100 μL of peripheral whole blood). (CD48 抗体 (FITC))

FACS

Surface staining of human peripheral blood cells with anti-CD48 (MEM-102) Alexa Fluor® 700. (CD48 抗体 (FITC))

FACS

Surface staining of human peripheral blood with anti-CD48 (MEM-102) FITC. (CD48 抗体 (FITC))

FACS

3 images

(5 references)

产品编号 ABIN94159

发货至: 中国

Datasheet as PDF

抗原 See all CD48 抗体

CD48
适用
87

46

14

6

5

4

4

2

1

1

1

1
人, Non-Human Primate
宿主
56

35

14

6

6
小鼠
Compatible Secondaries
克隆类型
81

33
单克隆
标记
48

12

11

11

7

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This CD48 antibody is conjugated to FITC
应用范围
76

43

19

15

15

13

11

6

6

3

1

1

1

1

1
Flow Cytometry (FACS)

特异性

The antibody MEM-102 reacts with CD48 (Blast-1), a 40-47 kDa GPI-anchored extracellular membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells, it is negative on granulocytes, platelets and erythrocytes.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Raji human Burkitt's lymphoma cell line

克隆位点

MEM-102

亚型

IgG1

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CD48 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-102 Biotin

anti-CD48 (CD48) (AA 27-220) antibody
CD48 适用: 人 FACS, WB, ELISA, IF 宿主: 小鼠 Monoclonal 6F9H2 unconjugated

anti-CD48 (CD48) antibody
CD48 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-102 unconjugated

anti-CD48 (CD48) antibody
CD48 适用: 人 WB 宿主: 兔 Monoclonal unconjugated

anti-CD48 (CD48) (AA 1-243) antibody
CD48 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-CD48 (CD48) (AA 1-169) antibody
CD48 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B8-2C2 unconjugated

anti-CD48 (CD48) (AA 27-220) antibody
CD48 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM43 unconjugated Recombinant Antibody

anti-CD48 (CD48) antibody (APC)
CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 APC

anti-CD48 (CD48) antibody (PE)
CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 PE

anti-CD48 (CD48) (AA 47-181) antibody
CD48 适用: 人 FACS, WB, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 3F5H10 unconjugated

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, (1999) (PubMed).

Korínek, Stefanová, Angelisová, Hilgert, Horejsí: "The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1." in: Immunogenetics, Vol. 33, Issue 2, pp. 108-12, (1991) (PubMed).

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, (1990) (PubMed).
抗原

CD48

别名

CD48 (CD48 产品)

别名

AI449234 antibody, AW610730 antibody, BCM1 antibody, BLAST antibody, BLAST-1 antibody, BLAST1 antibody, Bcm-1 antibody, MEM-102 antibody, SLAMF2 antibody, Sgp-60 antibody, hCD48 antibody, mCD48 antibody, CD48 antigen antibody, Cd48 molecule antibody, CD48 molecule antibody, Cd48 antibody, CD48 antibody

背景

CD48 Molecule,CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.,BCM1, BLAST, BLAST1, SLAMF2

基因ID

962

UniProt

P09326