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CD4 抗体 (N-Term) (PE)

Name: CD4 抗体 (N-Term) (PE)
Brand: antibodies-online
SKU: ABIN94091
Availability: OutOfStock

CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD4 (MEM-241) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD4 抗体 (N-Term) (PE))

FACS

Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) FITC. (CD4 抗体 (N-Term) (PE))

FACS

Separation of human CD4 positive lymphocytes (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD4 (MEM-241) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD4 抗体 (N-Term) (PE))

FACS

3 images

(6 references)

产品编号 ABIN94091

发货至: 中国

Datasheet as PDF

抗原 See all CD4 抗体

CD4
抗原表位
28

16

16

16

16

9

7

6

5

4

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
329

200

109

46

24

20

19

18

14

10

10

10

10

8

7

6

6

6

6

5

2

2

1

1

1

1
人
宿主
318

144

106

3

3

1
小鼠
Compatible Secondaries
克隆类型
432

138

3
单克隆
标记
185

66

57

46

34

14

12

10

10

9

9

8

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD4 antibody is conjugated to PE
应用范围
453

188

130

89

88

66

55

42

39

34

29

25

10

7

7

7

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

特异性

The antibody MEM-241 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“,helper“, T-cells) and also on monocytes, tissue macrophages and granulocytes.

交叉反应（详细）

Human, Other not tested

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

2 N-terminal domains of human CD4 fused to human IgG1 Fc

克隆位点

MEM-241

亚型

IgG1

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CD4 适用: 人 FACS, WB, CyTOF 宿主: 小鼠 Monoclonal MEM-241 unconjugated

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CD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC

anti-CD4 (CD4) antibody (PE)
CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Anderson, Sayers, Haniffa, Swan, Diboll, Wang, Isaacs, Hilkens: "Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 124-33, (2008) (PubMed).

Karlsson, Cowley, Martinez, Shaw, Minger, James: "Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3." in: Experimental hematology, Vol. 36, Issue 9, pp. 1167-75, (2008) (PubMed).

Manasa, Musabaike, Masimirembwa, Burke, Luthy, Mudzori: "Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe." in: Clinical and vaccine immunology : CVI, Vol. 14, Issue 3, pp. 293-8, (2007) (PubMed).

Zola, Swart, Banham, Barry, Beare, Bensussan, Boumsell, D Buckley, Bühring, Clark, Engel, Fox, Jin, Macardle, Malavasi, Mason, Stockinger, Yang: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).
抗原

CD4

别名

CD4 (CD4 产品)

别名

cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

背景

CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4

基因ID

920

UniProt

P01730

途径

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events