CD4 抗体 (N-Term) (Biotin)
(6 references)-
- 抗原 See all CD4 抗体
- CD4
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抗原表位
- N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD4 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB)
- 特异性
- The antibody MEM-241 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“,helper“, T-cells) and also on monocytes, tissue macrophages and granulocytes.
- 交叉反应 （详细）
- Human, Other not tested
- 纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
- 免疫原
- 2 N-terminal domains of human CD4 fused to human IgG1 Fc
- 克隆位点
- MEM-241
- 亚型
- IgG1
- Top Product
- Discover our top product CD4 Primary Antibody
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Pig, 犬, Cat, 兔, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠 IF, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 适用: 人 FACS, WB, CyTOF 宿主: 小鼠 Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) (AA 300-400) antibodyCD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE
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- 应用备注
- Flow cytometry: Recommended dilution: 1 μg/mL, positive control: peripheral blood, HPB T cell line. This antibody is excellent antibody for FC application.
- 说明
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The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).
: "Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 124-33, (2008) (PubMed).
: "Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3." in: Experimental hematology, Vol. 36, Issue 9, pp. 1167-75, (2008) (PubMed).
: "Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe." in: Clinical and vaccine immunology : CVI, Vol. 14, Issue 3, pp. 293-8, (2007) (PubMed).
: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, (2007) (PubMed).
: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).
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: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).
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- 抗原
- CD4
- 别名
- CD4 (CD4 产品)
- 别名
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- 背景
- CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4
- 基因ID
- 920
- UniProt
- P01730
- 途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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