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CD4 抗体 (N-Term)

Name: CD4 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN94088
Availability: OutOfStock

CD4 适用: 人 FACS, WB, CyTOF 宿主: 小鼠 Monoclonal MEM-241 unconjugated

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD4 (MEM-241) purified antibody (concentration in sample 1 μg/mL) GAM APC. (CD4 抗体 (N-Term))

FACS

Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) FITC. (CD4 抗体 (N-Term))

FACS

Mass cytometry (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD4 (MEM-241) Er166. (CD4 抗体 (N-Term))

CyTOF

Separation of human CD4 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD4 (MEM-241) purified antibody (concentration in sample 1 μg/mL) GAM APC. (CD4 抗体 (N-Term))

FACS

4 images

(7 references)

产品编号 ABIN94088

发货至: 中国

Datasheet as PDF

抗原 See all CD4 抗体

CD4
抗原表位
28

16

16

16

16

9

7

6

5

4

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
329

200

109

46

24

20

19

18

14

10

10

10

10

8

7

6

6

6

6

5

2

2

1

1

1

1
人
宿主
318

144

106

3

3

1
小鼠
Compatible Secondaries
克隆类型
432

138

3
单克隆
标记
184

66

58

46

34

14

12

10

10

9

9

8

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD4 antibody is un-conjugated
应用范围
453

187

130

89

88

66

55

42

39

34

29

25

10

7

7

7

6

6

5

3

3

3

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Cytometry by Time of Flight (CyTOF)

特异性

The antibody MEM-241 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“,helper“, T-cells) and also on monocytes, tissue macrophages and granulocytes.

交叉反应（详细）

Human, Other not tested

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

2 N-terminal domains of human CD4 fused to human IgG1 Fc

克隆位点

MEM-241

亚型

IgG1

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CD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE

应用备注

Flow cytometry: Recommended dilution: 1-5 μg/mL, positive control: peripheral blood, HPB T cell line. This antibody is excellent antibody for FC application.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: peripheral blood, HPB T cell line, non-reducing conditions.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Holmannova, Kolackova, Mandak, Kunes, Holubcova, Krejsek, Vlaskova, Andrys: "Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery." in: Perfusion, (2014) (PubMed).

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Anderson, Sayers, Haniffa, Swan, Diboll, Wang, Isaacs, Hilkens: "Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 124-33, (2008) (PubMed).

Karlsson, Cowley, Martinez, Shaw, Minger, James: "Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3." in: Experimental hematology, Vol. 36, Issue 9, pp. 1167-75, (2008) (PubMed).

Manasa, Musabaike, Masimirembwa, Burke, Luthy, Mudzori: "Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe." in: Clinical and vaccine immunology : CVI, Vol. 14, Issue 3, pp. 293-8, (2007) (PubMed).

Zola, Swart, Banham, Barry, Beare, Bensussan, Boumsell, D Buckley, Bühring, Clark, Engel, Fox, Jin, Macardle, Malavasi, Mason, Stockinger, Yang: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).
抗原

CD4

别名

CD4 (CD4 产品)

别名

cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

背景

CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4

基因ID

920

UniProt

P01730

途径

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events