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CD34 抗体 (PE)

Name: CD34 抗体 (PE)
Brand: antibodies-online
SKU: ABIN94080
Availability: OutOfStock

CD34 适用: 人 FACS, ICC 宿主: 小鼠 Monoclonal 4H11[APG] PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD34 抗体 (PE))

FACS

Flow Cytometry analysis Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. (CD34 抗体 (PE))

FACS

Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 PE. (CD34 抗体 (PE))

Immunocytochemistry detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE. (CD34 抗体 (PE))

ICC

Separation of human CD34 positive CD45dim stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD34 (4H11[APG]) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD34 抗体 (PE))

FACS

5 images

(1 reference)

产品编号 ABIN94080

发货至: 中国

Datasheet as PDF

抗原 See all CD34 抗体

CD34
适用
287

107

105

33

19

17

17

17

17

16

9

6

6

1

1
人
宿主
155

144

17

4
小鼠
Compatible Secondaries
克隆类型
198

119

2
单克隆
标记
120

27

24

20

14

8

6

6

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This CD34 antibody is conjugated to PE
应用范围
219

192

86

65

58

48

39

37

30

28

23

16

13

7

6

4

3

3

2

1

1
Flow Cytometry (FACS), Immunocytochemistry (ICC)

特异性

The mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

克隆位点

4H11[APG]

亚型

IgG1

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anti-CD34 (CD34) antibody
CD34 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), Func 宿主: 小鼠 Monoclonal QBEnd-10 unconjugated

anti-CD34 (CD34) (C-Term) antibody
CD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) (AA 151-385) antibody
CD34 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) (AA 124-375) antibody
CD34 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) antibody
CD34 适用: 人 FACS, WB, IHC (p), ICC 宿主: 小鼠 Monoclonal 4H11[APG] unconjugated

anti-CD34 (CD34) antibody (FITC)
CD34 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 581 FITC

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Elknerová, Lacinová, Soucek, Marinov, Stöckbauer: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, (2007) (PubMed).
抗原

CD34

别名

CD34 (CD34 产品)

别名

AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

背景

CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

基因ID

947

UniProt

P28906