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CD3 抗体 (PE)

CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 PE

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (MEM-57) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD3 抗体 (PE))

Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. (CD3 抗体 (PE))

Surface staining of CD3 in human peripheral blood with anti-CD3 (MEM-57) PE. (CD3 抗体 (PE))

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (MEM-57) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD3 抗体 (PE))

4 images

(9 references)

产品编号 ABIN94062

发货至: 中国

Datasheet as PDF

抗原 See all CD3 抗体

CD3
适用
177

94

24

20

14

10

8

8

7

5

3

3

2

1
人
宿主
209

44

19

13

8
小鼠
Compatible Secondaries
克隆类型
283

6
单克隆
标记
88

36

29

23

23

11

8

6

6

5

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is conjugated to PE
应用范围
266

82

56

53

44

37

30

23

10

7

7

6

4

3

2

2

2

2

2

2

2

1

1
Flow Cytometry (FACS)

特异性

The antibody MEM-57 reacts with an extracellular epitope on gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Human thymocytes and T lymphocytes.

克隆位点

MEM-57

亚型

IgG2a kappa

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anti-CD3 (CD3) antibody
CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody
CD3 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 适用: 人 FACS, IP, Func, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (APC)
CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC

anti-CD3 (CD3) antibody (PerCP)
CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PE)
CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, (2012) (PubMed).

Lesport, Baudhuin, Sousa, Lemaoult, Zamborlini, Rouas-Freiss, Carosella, Favier: "Inhibition of human Vγ9Vδ2 T-cell antitumoral activity through HLA-G: implications for immunotherapy of cancer." in: Cellular and molecular life sciences : CMLS, (2011) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Panyi, Bagdány, Bodnár, Vámosi, Szentesi, Jenei, Mátyus, Varga, Waldmann, Gáspar, Damjanovich: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, (2003) (PubMed).

Dave, Cao, Browne, Alarcon, Fernandez-Miguel, Lafaille, de la Hera, Tonegawa, Kappes: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." in: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, (1997) (PubMed).

Soucek, Hilgert, Budová, Lindnerová: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, (1994) (PubMed).

Hilgert, Fran?k, Stefanová, Kaslík, Jirka, Kristofová, Rossmann, Soucek, Horejsi: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." in: Transplantation, Vol. 55, Issue 2, pp. 435-8, (1993) (PubMed).

Soucek, Chudomel, Hrubá, Lindnerová: "Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3." in: Neoplasma, Vol. 38, Issue 1, pp. 33-41, (1991) (PubMed).
抗原

CD3

别名

CD3 (CD3 产品)

别名

CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

背景

CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

基因ID

916

UniProt

P07766

途径

TCR Signaling, Ubiquitin Proteasome Pathway

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