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TERT 抗体 (AA 597-611)

Verified TERT 适用: 人 ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN940568
发货至: 中国
  • 抗原 See all TERT 抗体
    TERT (Telomerase Reverse Transcriptase (TERT))
    抗原表位
    • 16
    • 16
    • 10
    • 10
    • 7
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 597-611
    适用
    • 102
    • 37
    • 20
    • 5
    • 1
    • 1
    宿主
    • 106
    • 5
    山羊
    克隆类型
    • 97
    • 14
    多克隆
    标记
    • 50
    • 8
    • 7
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This TERT antibody is un-conjugated
    应用范围
    • 66
    • 48
    • 37
    • 27
    • 17
    • 15
    • 13
    • 12
    • 8
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
    原理
    TERT (aa597-611)
    序列
    QLRELSEAEV RQHRE
    特异性
    This antibody is expected to recognize both reported isoforms (NP_937983.2, (NP_001180305.1).
    交叉反应
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-QLRELSEAEVRQHRE, from the internal region of the protein sequence according to NP_937983.2, NP_001180305.1.
    亚型
    IgG
    Top Product
    Discover our top product TERT Primary Antibody
  • 应用备注

    Western Blot: Preliminary testing showed a band at approx 125 kDa in lysates of cell lines A549 and CaCo-2 after 1 μg/mL antibody staining (calculated MW of 127 kDa according to NP_937983.2). Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    TERT (Telomerase Reverse Transcriptase (TERT))
    别名
    TERT (TERT 产品)
    背景
    TERT, telomerase reverse transcriptase, EST2, TCS1, TP2, TRT, hEST2, telomerase catalytic subunit, telomerase-associated protein 2
    基因ID
    7015
    NCBI登录号
    NP_937983, NP_001180305
    途径
    Telomere Maintenance
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