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CD25 抗体 (Biotin)

Name: CD25 抗体 (Biotin)
Brand: antibodies-online
SKU: ABIN94045
Availability: OutOfStock

IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 Biotin

Flow cytometry analysis (surface staining) of PHA-stimulated (3 days) human PBMC with anti-CD25 (MEM-181) biotin, streptavidin-APC. (CD25 抗体 (Biotin))

FACS

Surface staining of human PBMC with anti-human CD25 (MEM-181) FITC. (CD25 抗体 (Biotin))

FACS

2 images

(1 reference)

产品编号 ABIN94045

发货至: 中国

Datasheet as PDF

抗原 See all CD25 (IL2RA) 抗体

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
适用
175

111

32

9

6

3

3

2

1

1

1

1
人
宿主
129

85

77

2

1

1
小鼠
Compatible Secondaries
克隆类型
212

79

2
单克隆
标记
107

32

26

25

21

14

6

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD25 antibody is conjugated to Biotin
应用范围
206

85

52

49

39

36

19

16

14

14

8

8

6

4

4

3

2

2

1

1

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1
Flow Cytometry (FACS)

特异性

The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost on resting B and T lymphocytes.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

免疫原

PHA-activated peripheral blood leucocytes

克隆位点

MEM-181

亚型

IgG1

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anti-Interleukin 2 Receptor, alpha (IL2RA) antibody
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anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)
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应用备注

Flow cytometry: Recommended dilution: 1-2 μg/mL.

说明

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
抗原

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

别名

CD25 (IL2RA 产品)

别名

CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody

背景

Interleukin 2 receptor subunit alpha,CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 Molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.,IL-2R α, chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10, TCGFR

基因ID

3559

UniProt

P01589

途径

JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation