CD15 抗体
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北京 101111
Quick Overview for CD15 抗体 (ABIN93984)
抗原
See all CD15 (FUT4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD15 Purified
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特异性
- The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on the surface of granulocytes, monocytes, macrophages, mast cells, it is also present on Langerhans cells and some myeloid precursors cells.
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无交叉反应
- Cow, Pig, 绵羊
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交叉反应 （详细）
- Human
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纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Human granulocytes
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亚型
- IgM kappa
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anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 264-497) antibody
FUT4 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 144-389) antibodyFUT4 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 251-295) antibodyFUT4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p), ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 200-300) antibodyFUT4 适用: 人 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 199-302) antibodyFUT4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B11B4 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 1-147) antibodyFUT4 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6C5A3 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibodyFUT4 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal Leu-M1-same as MMA unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (FITC)FUT4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-158 FITC
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (Biotin)FUT4 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-158 Biotin
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (APC)FUT4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-158 APC
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应用备注
- Flow cytometry: Recommended dilution: 1-4 μg/mL.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
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别名
- CD15
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背景
- CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.,Lewis x Blood Group antigen, Le(x), SSEA-1, 3-fucosyl-N-acetyllactosamine
抗原
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