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CD11b 抗体 (PE)

Name: CD11b 抗体 (PE)
Brand: antibodies-online
SKU: ABIN93960
Availability: OutOfStock

ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 PE

Separation of human monocytes (red-filled) from CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11b (MEM-174) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD11b 抗体 (PE))

FACS

Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. (CD11b 抗体 (PE))

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD11b (MEM-174) PE. (CD11b 抗体 (PE))

FACS

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD11b (MEM-174) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (CD11b 抗体 (PE))

FACS

4 images

(2 references)

产品编号 ABIN93960

发货至: 中国

Datasheet as PDF

抗原 See all CD11b (ITGAM) 抗体

CD11b (ITGAM) (Integrin alpha M (ITGAM))
适用
258

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64

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人
宿主
141

105

104

2

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小鼠
Compatible Secondaries
克隆类型
257

94

1
单克隆
标记
120

39

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This CD11b antibody is conjugated to PE
应用范围
265

82

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59

46

35

22

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Flow Cytometry (FACS)

特异性

The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Human granulocytes

克隆位点

MEM-174

亚型

IgG2a

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应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, (2015) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
抗原

CD11b (ITGAM) (Integrin alpha M (ITGAM))

别名

CD11b (ITGAM 产品)

背景

Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

基因ID

3684

UniProt

P11215

途径

Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation