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- 抗原 See all ACRV1 抗体
- ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
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适用
- 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ACRV1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC)
- 特异性
- The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
- 交叉反应 (详细)
- Canine (Dog)
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
- 克隆位点
- Ds-2
- 亚型
- IgG
- Top Product
- Discover our top product ACRV1 Primary Antibody
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- 应用备注
- Immunocytochemistry: Membrane permeabilization (acetone) is essential.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." in: Andrologia, Vol. 29, Issue 5, pp. 261-8, (1997) (PubMed).
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Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." in: Andrologia, Vol. 29, Issue 5, pp. 261-8, (1997) (PubMed).
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- 抗原
- ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))
- 别名
- Intra-Acrosomal Protein (ACRV1 产品)
- 别名
- ACRV1 antibody, D11S4365 antibody, SP-10 antibody, SPACA2 antibody, Msa63 antibody, Sp10 antibody, acrosomal vesicle protein 1 antibody, ACRV1 antibody, Acrv1 antibody
- 背景
- One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,IAP Can
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