alpha Tubulin 抗体
(3 references)Quick Overview for alpha Tubulin 抗体 (ABIN93894)
抗原
See all alpha Tubulin (TUBA1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-alpha-Tubulin Purified
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特异性
- The antibody TU-16 reacts with alpha-tubulin of all tested species, under denaturing and non-denaturing conditions.
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交叉反应 （详细）
- Broad species reactivity
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纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Porcine brain microtubule protein MTP-1.
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亚型
- IgM
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anti-alpha Tubulin (TUBA1) antibody
TUBA1 适用: 人, 小鼠, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Eisenia fetida, Turkey, Paramecium WB, IHC (p), IP, ICC, ICFC 宿主: 小鼠 Monoclonal TU-01 unconjugated
anti-alpha Tubulin (TUBA1) antibody (FITC)TUBA1 适用: 人, 小鼠, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Eisenia fetida, Turkey, Paramecium ICC, FACS 宿主: 小鼠 Monoclonal TU-01 FITC
anti-alpha Tubulin (TUBA1) antibody (Biotin)TUBA1 适用: 人, 小鼠, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Eisenia fetida, Turkey, Paramecium WB, IHC (p), ICC, ICFC 宿主: 小鼠 Monoclonal TU-01 Biotin
anti-alpha Tubulin (TUBA1) (acLys40) antibodyTUBA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-alpha Tubulin (TUBA1) antibodyTUBA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha Tubulin (TUBA1) antibodyTUBA1 适用: 人, 小鼠, 大鼠, Pig, Cow, Saccharomyces cerevisiae, 猴, 犬, Hamster, 豚鼠, Sea Urchin, Amphibian, 山羊, Kangaroo WB, IHC, IHC (p), IP 宿主: 小鼠 Monoclonal DM1A unconjugated
anti-alpha Tubulin (TUBA1) (Subunit alpha) antibodyTUBA1 适用: 人, Saccharomyces cerevisiae, 哺乳动物, Avian, Drosophila melanogaster, Fungus WB, ELISA, IHC, IHC (p), IHC (fro), RIA 宿主: 大鼠 Monoclonal YOL1-34 unconjugated
anti-alpha Tubulin (TUBA1) antibodyTUBA1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha Tubulin (TUBA1) (AA 419-435) antibodyTUBA1 适用: 人, 小鼠, 大鼠, Saccharomyces cerevisiae, Vertebrate, Invertebrate WB, ELISA, IP, ICC 宿主: 小鼠 Monoclonal 3A2 unconjugated
anti-alpha Tubulin (TUBA1) (AA 375-448) antibodyTUBA1 适用: 人, 小鼠, 大鼠, 植物 WB, ELISA, IHC (p), FACS, IF (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL.
Immunoprecipitation: Reducing conditions.
Western blotting: Recommended dilution: 1-2 μg/mL. This antibody can be used for Western blotting, but its alternative TU-02 (11-447-C100) gives better signal in this application. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut." in: Journal of neurobiology, Vol. 64, Issue 2, pp. 157-69, (2005) (PubMed).
: "Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila." in: Molecular biology of the cell, Vol. 15, Issue 11, pp. 4854-65, (2004) (PubMed).
: "Novel monoclonal antibodies TU-08 and TU-16 specific for tubulin subunits." in: Folia biologica, Vol. 44, Issue 1, pp. 35-6, (2000) (PubMed).
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: "D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut." in: Journal of neurobiology, Vol. 64, Issue 2, pp. 157-69, (2005) (PubMed).
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- alpha Tubulin (TUBA1)
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别名
- alpha-Tubulin
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背景
- Tubulin alpha 1,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.,TUBA
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基因ID
- 7277
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UniProt
- Q71U36
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途径
- Microtubule Dynamics
抗原
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