我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

alpha Tubulin 抗体

Name: alpha Tubulin 抗体
Brand: antibodies-online
SKU: ABIN93891
Availability: OutOfStock

TUBA1 适用: 人, 小鼠, Pig, Saccharomyces cerevisiae, Nicotiana tabacum, Arabidopsis, Turkey, Paramecium, Eisenia fetida WB, IHC (p), IP, ICC, FACS 宿主: 小鼠 Monoclonal TU-01 unconjugated

Anti-alpha-Tubulin Purified (TU-01) works in WB application under reducing conditions. (alpha Tubulin 抗体)

Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (; red). (alpha Tubulin 抗体)

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01 ; green) and anti-Vimentin (VI-01; red). (alpha Tubulin 抗体)

Nucleus is stained with DAPI (blue). (alpha Tubulin 抗体)

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B). (alpha Tubulin 抗体)

Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. (alpha Tubulin 抗体)

Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01, green) and anti-Vimentin (VI-01, cat. (alpha Tubulin 抗体)

13 images

(20 references)

产品编号 ABIN93891

发货至: 中国

Datasheet as PDF

抗原 See all alpha Tubulin (TUBA1) 抗体

alpha Tubulin (TUBA1)
适用
102

76

68

21

19

16

14

11

8

5

5

4

4

4

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
人, 小鼠, Pig, Saccharomyces cerevisiae, Nicotiana tabacum, Arabidopsis, Turkey, Paramecium, Eisenia fetida
宿主
56

52

8

2

2

1

1

1
小鼠
Compatible Secondaries
克隆类型
68

52

2
单克隆
标记
85

11

6

3

2

2

2

2

2

2

2

1

1

1

1
This alpha Tubulin antibody is un-conjugated
应用范围
97

45

38

35

33

33

25

14

7

7

4

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

特异性

The antibody TU-01 recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.

交叉反应（详细）

Broad species reactivity

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.

克隆位点

TU-01

亚型

IgG1
anti-alpha Tubulin (TUBA1) antibody (FITC)
TUBA1 适用: 人, 小鼠, Pig, Saccharomyces cerevisiae, Nicotiana tabacum, Arabidopsis, Turkey, Paramecium, Eisenia fetida ICC, FACS 宿主: 小鼠 Monoclonal TU-01 FITC

anti-alpha Tubulin (TUBA1) antibody (Biotin)
TUBA1 适用: 人, 小鼠, Pig, Saccharomyces cerevisiae, Nicotiana tabacum, Arabidopsis, Turkey, Paramecium, Eisenia fetida WB, IHC (p), ICC, FACS 宿主: 小鼠 Monoclonal TU-01 Biotin

anti-alpha Tubulin (TUBA1) antibody
TUBA1 适用: 人, 小鼠, 大鼠, Pig, Nicotiana tabacum, 犬 WB, ELISA, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal TU-16 unconjugated

anti-alpha Tubulin (TUBA1) (C-Term) antibody
TUBA1 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA, IHC, FM 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated

anti-alpha Tubulin (TUBA1) (Internal Region) antibody
TUBA1 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) (AA 380-460) antibody
TUBA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) (acLys40) antibody
TUBA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) antibody
TUBA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) antibody
TUBA1 适用: 人, 小鼠, 大鼠, Pig, Cow, Saccharomyces cerevisiae, 猴, Hamster, 犬, 豚鼠, 山羊, Sea Urchin, Amphibian, Kangaroo WB, IHC, IHC (p), IP 宿主: 小鼠 Monoclonal DM1A unconjugated

anti-alpha Tubulin (TUBA1) (Subunit alpha) antibody
TUBA1 适用: 人, Saccharomyces cerevisiae, 哺乳动物, Avian, Drosophila melanogaster, Fungus WB, ELISA, IHC, IHC (p), IHC (fro), RIA 宿主: 大鼠 Monoclonal YOL1-34 unconjugated

应用备注

Flow cytometry: Recommended dilution: 1-4 μg/mL, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Peskova, Cerna, Oppelt, Mraz, Barta: "Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts." in: Scientific reports, Vol. 9, Issue 1, pp. 15759, (2019) (PubMed).

Bohaciakova, Renzova, Fedorova, Barak, Kunova Bosakova, Hampl, Cajanek: "An Efficient Method for Generation of Knockout Human Embryonic Stem Cells Using CRISPR/Cas9 System." in: Stem cells and development, Vol. 26, Issue 21, pp. 1521-1527, (2018) (PubMed).

Lueck, Hennig, Lommatzsch, Pauleikhoff, Wasmuth: "Complement and UV-Irradiated Photoreceptor Outer Segments Increase the Cytokine Secretion by Retinal Pigment Epithelial Cells." in: Investigative ophthalmology & visual science, Vol. 53, Issue 3, pp. 1406-13, (2012) (PubMed).

Dolezalova, Mraz, Barta, Plevova, Vinarsky, Holubcova, Jaros, Dvorak, Pospisilova, Hampl: "MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 7, pp. 1362-72, (2012) (PubMed).

Pospichalova, Tureckova, Fafilek, Vojtechova, Krausova, Lukas, Sloncova, Takacova, Divoky, Leprince, Plachy, Korinek: "Generation of two modified mouse alleles of the Hic1 tumor suppressor gene." in: Genesis (New York, N.Y. : 2000), (2011) (PubMed).

Krupkova, Loja, Redova, Neradil, Zitterbart, Sterba, Veselska: "Analysis of nuclear nestin localization in cell lines derived from neurogenic tumors." in: Tumour biology, (2011) (PubMed).

Sana, Zambo, Skoda, Neradil, Chlapek, Hermanova, Mudry, Vasikova, Zitterbart, Hampl, Sterba, Veselska: "CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines." in: Analytical cellular pathology (Amsterdam), (2011) (PubMed).

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Buarta, Vinarskuy, Holubcovua, Dole?alovua, Verner, Pospui?ilovua, Dvo?uak, Hampl: "Human Embryonic Stem Cells are Capable of Executing G1/S Checkpoint Activation." in: Stem cells (Dayton, Ohio), (2010) (PubMed).

Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." in: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, (2009) (PubMed).

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, (2004) (PubMed).

Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, (1997) (PubMed).

Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

Linhartová, Dráber, Dráberová, Viklický: "Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins." in: The Biochemical journal, Vol. 288 ( Pt 3), pp. 919-24, (1993) (PubMed).

Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." in: Histochemistry, Vol. 95, Issue 5, pp. 519-24, (1991) (PubMed).

Dráber, Dráberová, Linhartová, Viklický: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." in: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, (1990) (PubMed).

Dráber, Dráberová, Zicconi, Sellitto, Viklický, Cappuccinelli: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." in: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, (1987) (PubMed).

Grimm, Breitling, Little: "Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1." in: Biochimica et biophysica acta, Vol. 914, Issue 1, pp. 83-8, (1987) (PubMed).

Viklický, Dráber, Hasek, Bártek: "Production and characterization of a monoclonal antitubulin antibody." in: Cell biology international reports, Vol. 6, Issue 8, pp. 725-31, (1982) (PubMed).

show more references
抗原

alpha Tubulin (TUBA1)

别名

alpha-tubulin (TUBA1 产品)

别名

Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody

背景

Tubulin alpha 1,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.,TUBA

基因ID

7277

UniProt

Q71U36

途径

Microtubule Dynamics