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alpha Fetoprotein 抗体

AFP 适用: 人 WB, ELISA, IP, ICC 宿主: 小鼠 Monoclonal AFP-01 unconjugated
产品编号 ABIN93889
发货至: 中国
  • 抗原 See all alpha Fetoprotein (AFP) 抗体
    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    This alpha Fetoprotein antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
    特异性
    The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
    交叉反应 (详细)
    Other not tested, Human
    纯化方法
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    purified alpha-Fetoprotein
    克隆位点
    AFP-01
    亚型
    IgG1
    Top Product
    Discover our top product AFP Primary Antibody
  • 应用备注
    ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).
    Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions.
    Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

  • 抗原
    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
    别名
    alpha-Fetoprotein (AFP 产品)
    别名
    FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
    背景
    Alpha fetoprotein,Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.,AFP, FETA, HPAFP
    基因ID
    174
    UniProt
    P02771
    途径
    C21-Steroid Hormone Metabolic Process
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