alpha Fetoprotein 抗体
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- 抗原 See all alpha Fetoprotein (AFP) 抗体
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This alpha Fetoprotein antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
- 特异性
- The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
- 交叉反应 (详细)
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- purified alpha-Fetoprotein
- 克隆位点
- AFP-11
- 亚型
- IgG1
- Top Product
- Discover our top product AFP Primary Antibody
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- 应用备注
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ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: hepatocellular carcinoma, heat mediated antigen retrieval (sodium citrate buffer), mAb incubation 1 hour / RT, detection DAB.
Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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- 抗原
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- 别名
- alpha-Fetoprotein (AFP 产品)
- 别名
- FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
- 背景
- Alpha fetoprotein,Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.,AFP, FETA, HPAFP
- 基因ID
- 174
- UniProt
- P02771
- 途径
- C21-Steroid Hormone Metabolic Process
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