CLNS1A 抗体
(2 validations)-
- 抗原 See all CLNS1A 抗体
- CLNS1A (Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A))
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适用
- 人
-
宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CLNS1A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB)
- 交叉反应
- 人, 小鼠, 大鼠
- 交叉反应 （详细）
- Other species not studied.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- CLNS1 A antibody was raised in mouse using recombinant Human Chloride Channel, Nucleotide-Sensitive, 1 (Clns1 )
- 克隆位点
- 2187C2a
- 亚型
- IgG1
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anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (AA 1-237) antibody
CLNS1A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (AA 189-216), (C-Term) antibodyCLNS1A 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19567 unconjugated
anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (AA 148-176) antibodyCLNS1A 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19566 unconjugated
anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (C-Term) antibodyCLNS1A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (C-Term) antibodyCLNS1A 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (AA 138-187) antibodyCLNS1A 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, Hamster, 猴, Bat, Pig, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (C-Term) antibodyCLNS1A 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (C-Term) antibodyCLNS1A 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: 0.2-2 µg/mL, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator. - 限制
- 仅限研究用
-
- by
- AG Gruss, Institut für Genetik, Rheinische Friedrich-Wilhelms-Universität Bonn
- No.
- #102907
- 日期
- 2018.09.20
- 抗原
- CLNS1A
- Lot Number
- 9832
- Method validated
- Western Blotting
- Positive Control
HeLa cells
Independent antibody
- Negative Control
HeLa cells, CLNS1A knockdown using Dharmacon smartpool siRNA
- Notes
Passed. ABIN933127 specifically reveals endogenous CLNS1A in HeLa cell lysates while no signal is detected subsequently to knock-down of the antigen.
- Primary Antibody
- ABIN933127
- Secondary Antibody
- goat anti-mouse IgG (H+L) (Jackson ImmunoResearch, 115-005-003, lot 128525)
- Full Protocol
- Seed 3x104 HeLa cells in DMEM (PAN Biotech, P04-41450, 2730618) supplemented with 10% FBS (PAN Biotech, P03-3302), without antibiotics, in 6 well plates in 2 ml media.
- Grow for 24h cells at 37°C and 5% CO2 to 70% confluency.
- Change medium.
- Transfect cells with 60pmol ON-TARGETplus SmartPool siRNA human (Dharmacon, L-012571-00-0005, lot 170831) in 125µl 150mM NaCl added to 7.5µl Lipofectamine RNAiMax (13778030, lot 1911816) in 125µl 150mM NaCl.
- Grow cells for 24h at 37°C and 5% CO2.
- Change medium.
- Grow cells for 72h at 37°C and 5% CO2.
- Wash cells once with PBS (PAN Biotech, P04-36500, lot 3620618).
- Detach cells with 100µl trypsin per well.
- For WB the cells were once washed with PBS (PAN Biotech) before treated with 100µl of Trypsin (PAN Biotech, P10-023100, lot 6860417) per well.
- Once cells are detached add 900µl medium.
- Count cells.
- Add 40µl 1x Laemmli buffer (Roth, K929.1, lot 227259243) to 2x105 cells. Heat up to 99°C for 10min.
- Separate 10µl of the lysates on a freshly cast denaturing 12% SDS-PAGE gel in an electrophoresis chamber for 1h at 120V.
- Transfer proteins onto PVDF membrane (Merck, IPVH0010, lot K5KN7128I) with a wet blotting system for 1h at 80V.
- Block the membrane with 1x Roti Block (Roth, A151.2, lot 148270222) for 30min at RT.
- Incubation with primary
- mouse anti-CLNS1A antibody (antibodies-online, ABIN933127, lot 9832) diluted 1:500 in 1x Roti Block for 1h at RT,
- mouse anti-CLNS1A antibody (Santa Cruz, sc-271454, G3010) diluted 1:3000 in 1x Roti Block for 1h at RT, or
- rat anti tubulin antibody (YOL 1/34 gifted) diluted 1:1000 in 1x Roti Block for 1h at RT.
- Wash membrane 2x for 10min with 1x TBST.
- Incubation with secondary
- goat anti-mouse IgG (H+L) (Jackson ImmunoResearch, 115-005-003, lot 128525) diluted 1:10000 in PBS for 45min at RT or
- goat anti-rat IgG (H+L) (Jackson ImmunoResearch, 112-035-003) diluted 1:10000 in PBS for 45min at RT.
- Wash membrane 5x for 5min with 1x TBS-T.
- Reveal protein bands using ECL (GE Healthcare, RPN3244V2, 9633722) on an LAS mini system (GE healthcare).
- Experimental Notes
Western blot using ABIN933127 on HeLa cell extracts reveals a band of the expected molecular weight. The same band is also detected by an independent CLNS1A antibody and disappears upon siRNA knock-down.
生效 #102907 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Western blot analysis of HeLa cell lysates without (HeLa) or with siRNA knock-down (HeLa/kd). CLNS1A was specifically detected using ABIN933127 (A) and an independent antibody (B). Tubulin serves as loading control (C).]()
Western blot analysis of HeLa cell lysates without (HeLa) or with siRNA knock-down (HeLa/kd). CLNS1A was specifically detected using ABIN933127 (A) and an independent antibody (B). Tubulin serves as loading control (C).
Full Methods -
- by
- AG Gruss, Institut für Genetik, Rheinische Friedrich-Wilhelms-Universität Bonn
- No.
- #103452
- 日期
- 2018.09.20
- 抗原
- CLNS1A
- Lot Number
- 9832
- Method validated
- Immunofluorescence
- Positive Control
HeLa cells
Independent antibody
- Negative Control
HeLa cells, CLNS1A knockdown using Dharmacon smartpool siRNA
- Notes
Passed. ABIN933127 specifically reveals endogenous CLNS1A in HeLa cells while no signal is detected subsequently to knock-down of the antigen.
- Primary Antibody
- ABIN933127
- Secondary Antibody
- AF488 conjugated secondary goat anti-mouse IgG (H+L) (ThermoFisher Scientific, A32723)
- Full Protocol
- Seed 3x104 HeLa cells in DMEM (PAN Biotech, P04-41450, 2730618) supplemented with 10% FBS (PAN Biotech, P03-3302), without antibiotics, in 6 well plates in 2ml media.
- Grow cells for 24h at 37°C and 5% CO2 to 70% confluency.
- Change medium.
- Transfect cells with 60pmol ON-TARGETplus SmartPool siRNA human (Dharmacon, L-012571-00-0005, lot 170831) in 125µl 150mM NaCl added to 7.5µl Lipofectamine RNAiMax (13778030, lot 1911816) in 125µl 150mM NaCl.
- Grow cells for 24h at 37°C and 5% CO2.
- Change medium.
- Grow cells for 72h at 37°C and 5% CO2.
- Transfer cells from each well on five coverslips.
- Grow cells for 96h at 37°C and 5% CO2.
- Wash cells with 1x PBS (PAN Biotech).
- Add 4% PFA for 10min at 37°C.
- Wash cells 2x with 1x PBS 1x.
- Wash cells 1x 5min with 0.5% Triton X-100.
- Wash cells 2x with 1x PBS containing 1x Roti-Immunoblock (Roth, T144.1, lot 248272501).
- Store cells ON at 4°C.
- Incubation with primary
- mouse anti-CLNS1A antibody (antibodies-online, ABIN933127, lot 9832) diluted 1:500 in Roti-Immunoblock for 1h at RT or
- mouse anti-CLNS1A antibody (Santa Cruz, sc-271454, G3010) diluted 1:500 in Roti-Immunoblock for 1h at RT.
- Wash coverslips with PBS.
- Incubation with AF488 conjugated secondary goat anti-mouse IgG (H+L) (ThermoFisher Scientific, A32723) diluted 1:1000 for 45min at RT.
- Wash coverslips with PBS.
- Mount coverslips on glass slides in Fluoromount-G mounting medium (Southern Biotech, 0100-01).
- Image acquisition with an exposure time of 600ms (ABIN933127) or 400ms (sc-271454) on (Zeiss Axiophot, 63x magnification, resolution 1388x1040 pixels).
- Experimental Notes
IF staining of HeLA cells using ABIN933127 reveals the expected staining pattern. Cellular staining was not observed after CLNS1A knock-down.
生效 #103452 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Immunofluorescence labeling of CLNS1A in HeLa cells (HeLa) using ABIN933127 (A) or an independent antibody (B). The signal disappears after CLNS1A knock-down (HeLa/kd).]()
Immunofluorescence labeling of CLNS1A in HeLa cells (HeLa) using ABIN933127 (A) or an independent antibody (B). The signal disappears after CLNS1A knock-down (HeLa/kd).
Full Methods -
- 浓度
- Lot specific
- 缓冲液
- CLNS1 A antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
-
Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.
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- 抗原
- CLNS1A (Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A))
- 别名
- CLNS1A (CLNS1A 产品)
- 别名
- CLCI antibody, CLNS1B antibody, ICln antibody, 2610036D06Rik antibody, 2610100O04Rik antibody, Clci antibody, Clcni antibody, ICLN antibody, Icln antibody, pICln antibody, clci antibody, clns1b antibody, icln antibody, pIcIn antibody, chloride nucleotide-sensitive channel 1A antibody, chloride channel, nucleotide-sensitive, 1A antibody, chloride channel, nucleotide-sensitive, 1A L homeolog antibody, CLNS1A antibody, Clns1a antibody, clns1a.L antibody, clns1a antibody
- 背景
- The interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway. Synonyms: Monoclonal CLNS1A antibody, Anti-CLNS1A antibody, Chloride channel nucleotide sensitive 1A antibody, CLCI antibody, ICln antibody, CLNS1B antibody.
- 途径
- Ribonucleoprotein Complex Subunit Organization
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