Vimentin 抗体
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- 抗原 See all Vimentin (VIM) 抗体
- Vimentin (VIM)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Vimentin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells, and has a molecular weight of 57 kDa. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments. The antibody stains the 57 kDa vimentin band in immunoblots performed on a lysate of normal human lymphocytes. No detectable cross-reactivity with GFAP, Keratin, Neurofilament or Desmin.
- 纯化方法
- Purified
- 克隆位点
- V9
- 亚型
- IgG1
- Top Product
- Discover our top product VIM Primary Antibody
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- 应用备注
- Immunohistochemistry: For use on frozen sections and formalin-fixed/paraffin- embedded tissues if pre-treated with trypsin. Paraffin sections: Use at an approximate dilution of 1:50 - 1:100 in PBS. Trypsin digestion may be required. Frozen sections: Use at an approximate dilution of 1:300 in PBS.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 1 mL of purified monoclonal antibody at approximately 30 µg/mL in PBS containing 20 mM sodium azide, and protein stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- Vimentin (VIM)
- 别名
- Vimentin (VIM 产品)
- 途径
- Caspase Cascade in Apoptosis
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