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P-Glycoprotein 抗体

适用: 人, Hamster WB, IHC, FACS 宿主: 小鼠 Monoclonal JSB-1 unconjugated
产品编号 ABIN920710
发货至: 中国
  • 抗原 See all P-Glycoprotein products
    P-Glycoprotein
    适用
    • 20
    • 10
    • 9
    • 1
    人, Hamster
    宿主
    • 16
    • 5
    小鼠
    克隆类型
    • 13
    • 7
    • 1
    单克隆
    标记
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This P-Glycoprotein antibody is un-conjugated
    应用范围
    • 10
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    特异性
    JSB-1 reacts with a conserved cytoplasmic epitope of the human plasma membrane- associated 170-180 kDa glycoprotein, the expression of which is strongly correlated with the degree of multidrug resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas.
    产品特性
    Mouse myeloma (SP2/0) cells fused with immunized mouse (Balb/C) lymph node cells.
    过滤
    0.22 μm filtered
    克隆位点
    JSB-1
    亚型
    IgG1
  • 应用备注
    Flow cytometry: Use at least a 1:10 dilution. Fix cells in 10% (v/v) Lysing Solution, followed by primary antibody and anti-mouse- FITC. Immunocytochemistry: Use at least a 1:20 dilution. (acetone-fixed cell preparation) Immunohistochemistry: acetone-fixed frozen sections or formalin-fixed, paraffin-embedded tissues: Use at a 1:20 dilution. Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used (see below), paraffin-embedded tissue can also be stained with the antibody. Western blot: Start optimizing working dilution at 1:10, followed by incubation with anti- mouse HRP. B-5 Fixative Procedure: Fix tissue in freshly prepared B-5 fixative: add 2 mL of 40% formalin to 20 mL of B-5 Stock Solution. Sections must be dezenkerized to remove mercuric pigment before immunostaining. Dezenkerize by placing slides with sections in Gram's Iodine solution for 5 min. at room temperature. Wash well in distilled water. Place slides in Sodium Thiosulphate solution for 5 min. at room temperature. Wash in running tap water for 3 min. Place in PBS for further immunochemical procedures. B-5 Stock Solution: 12 g mercuric chloride 2.5 g sodium acetate Dissolve in 200 mL distilled water. Gram's Iodine: 1 g I2 2 g KI Dissolve in 300 mL distilled water. KAMIYA BIOMEDICAL COMPANY Rev. 021507 PRODUCT DATA SHEET KAMIYA BIOMEDICAL COMPANY, Seattle, WA USA Tel: (206) 575-8068
    Fax: (206) 575-8094 www.k-assay.com LifeScience@k-assay.com
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    1 mL (200 tests) or 0.5 mL (100 tests) monoclonal antibody at ~250 µg IgG/mL in tissue culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 µm filter. Culture medium: RPMI-1640, supplemented with Nutridoma-SR. The medium does not contain serum or added enzymes.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    P-Glycoprotein
    别名
    MDR1 / P-Glycoprotein (P-Glycoprotein 产品)
    别名
    ABC20 antibody, CD243 antibody, CLCS antibody, GP170 antibody, MDR1 antibody, P-GP antibody, PGY1 antibody, Abcb1 antibody, Mdr1a antibody, ABCB1 antibody, ABCB4 antibody, CMDR1 antibody, xemdr antibody, ATP binding cassette subfamily B member 1 antibody, ATP binding cassette subfamily B member 1A antibody, ATP binding cassette subfamily B member 1 L homeolog antibody, ABCB1 antibody, Abcb1a antibody, abcb1.L antibody
    背景
    Synonyms: P-glycoprotein, MDR1, p170, ABCB1
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