P-Glycoprotein 抗体
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- 抗原 See all P-Glycoprotein products
- P-Glycoprotein
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适用
- 人, Hamster
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This P-Glycoprotein antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 特异性
- JSB-1 reacts with a conserved cytoplasmic epitope of the human plasma membrane- associated 170-180 kDa glycoprotein, the expression of which is strongly correlated with the degree of multidrug resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas.
- 克隆位点
- JSB-1
- 亚型
- IgG1
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anti-P-Glycoprotein antibody
适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-P-Glycoprotein antibody适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-P-Glycoprotein (AA 647-677) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-P-Glycoprotein (AA 647-677) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-P-Glycoprotein antibody适用: 人, Hamster WB, IHC, FACS 宿主: 小鼠 Monoclonal JSB-1 unconjugated
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- 应用备注
- Can be used for the detection of MDR cells in human tumor samples. Flow Cytometry: Use at least 1:10. Fix cells in 10% (v/v) Lysing Solution, followed by primary antibody and anti-mouse-FITC. Immunocytochemistry: Use at least 1:20 (acetone fixed cell preparation) Immunohistochemistry: acetone-fixed frozen sections or formalin-fixed paraffin embedded tissues: Use at 1:20 dilution. Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used, paraffin-embedded tissue can also be stained with the antibody. Western blotting: Start optimizing working dilution at 1:10, followed by incubation with anti-mouse HRP.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 1 ml concentrated tissue culture supernatant (200 tests) at ~250 µg IgG/ml, with 1% BSA and 0.1% NaN3.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- P-Glycoprotein
- Abstract
- P-Glycoprotein 产品
- 别名
- ABC20 antibody, CD243 antibody, CLCS antibody, GP170 antibody, MDR1 antibody, P-GP antibody, PGY1 antibody, Abcb1 antibody, Mdr1a antibody, ABCB1 antibody, ABCB4 antibody, CMDR1 antibody, xemdr antibody, ATP binding cassette subfamily B member 1 antibody, ATP binding cassette subfamily B member 1A antibody, ATP binding cassette subfamily B member 1 L homeolog antibody, ABCB1 antibody, Abcb1a antibody, abcb1.L antibody
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