Immunohistochemistry: For acetone-fixed frozen sections, use at a 1:20 dilution followed by incubation with rabbit anti-rat Ig and a monoclonal rat APAAP complex. Alternatively, after incubation with M7I-3 at a 1:20 dilution and washing, slides can be incubated with biotinylated rabbit anti-rat Ig and streptavidin conjugated to horseradish peroxidase. Immunocytochemistry: For acetone-fixed cytospin preparations, use at a 1:20-1:50 dilution. Western blot: Use at a 1:20-1:50 dilution followed by anti-rat-HRP. Flow cytometry: Optimal conditions still to be determined. MRP7 expression as determined by MRP7 clone M7I-3. HEK cells HEK cells transfected with MRP7
限制
仅限研究用
状态
Liquid
缓冲液
1 mL (>200 tests) monoclonal antibody at 250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
抗原
ABCC10
(ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 10 (ABCC10))