Reacts specifically with the human Fas antigen, although the epitope has not been mapped. Binds both the soluble and membrane-bound forms of Fas. Does not react with mouse or gibbon Fas. Recognizes activated T-cells and a subpopulation of B-cells. The cell-surface Fas antigen is a 48 kDa transmembrane protein that can mediate apoptosis/programmed cell death and belongs to the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. The human Fas antigen is expressed in various human cells, including activated peripheral T and B lymphocytes, Various tumor cell lines, leukemic T cells, and myeloid cells. It is also differentially expressed on human thymocytes during thymic maturation.
产品特性
Mouse myeloma (P3.X63.Ag8.653) x immunized mouse (Balb/c) spleen cells.
纯化方法
Purified by protein A chromatography
纯度
> 95 % by SDS-PAGE.
免疫原
Human B lymphoblast cell line SKW6.4 cell surface molecules
Immunohistochemistry on cryostat sections and on cytospin preparations. Not suitable for use on paraffin sections. Flow Cytometry: Use at 1:20 dilution (as determined on SKW6.4 cells.
限制
仅限研究用
缓冲液
FITC-labeled antibody in 1 ml PBS, pH 7.4 with 1% BSA and 0.02% NaN3.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.