RAG2 抗体 (AA 451-527)
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- 抗原 See all RAG2 抗体
- RAG2 (Recombination Activating Gene 2 (RAG2))
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抗原表位
- AA 451-527
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RAG2 antibody is un-conjugated
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应用范围
- ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 预测反应
- Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human RAG2
- 亚型
- IgG
- Top Product
- Discover our top product RAG2 Primary Antibody
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anti-Recombination Activating Gene 2 (RAG2) (Center) antibody
RAG2 适用: 人, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 1-527) antibodyRAG2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 428-527) antibodyRAG2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G8 unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 258-527) antibodyRAG2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 498-527), (C-Term) antibodyRAG2 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 1-527) antibodyRAG2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (N-Term) antibodyRAG2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 350-527) antibodyRAG2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4D5 unconjugated
anti-Recombination Activating Gene 2 (RAG2) (AA 296-415) antibodyRAG2 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Recombination Activating Gene 2 (RAG2) antibodyRAG2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- RAG2 (Recombination Activating Gene 2 (RAG2))
- 别名
- RAG2 (RAG2 产品)
- 别名
- RAG-2 antibody, Rag-2 antibody, rag-2 antibody, zgc:136743 antibody, recombination activating 2 antibody, recombination activating gene 2 antibody, recombination activating gene 2 S homeolog antibody, RAG2 antibody, Rag2 antibody, rag2 antibody, rag2.S antibody
- 背景
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Synonyms: RAG 2, RAG-2, RAG2, RAG2_HUMAN, Recombination activating gene 2, VDJ recombination activating protein 2, VDJ recombination-activating protein 2.
Background: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.
- 基因ID
- 5897
- 途径
- Chromatin Binding, Production of Molecular Mediator of Immune Response
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