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CCDC42 抗体 (AA 221-312)

CCDC42 适用: 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN872760
发货至: 中国
  • 抗原 See all CCDC42 抗体
    CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
    抗原表位
    • 14
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 1
    • 1
    AA 221-312
    适用
    • 24
    • 23
    • 16
    • 1
    • 1
    • 1
    • 1
    • 1
    小鼠, 大鼠
    宿主
    • 38
    克隆类型
    • 38
    多克隆
    标记
    • 8
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CCDC42 antibody is un-conjugated
    应用范围
    • 32
    • 21
    • 14
    • 12
    • 12
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应
    小鼠, 大鼠
    预测反应
    Human,Dog,Cow,Sheep,Pig,Horse,Rabbit
    纯化方法
    Purified by Protein A.
    免疫原
    KLH conjugated synthetic peptide derived from human CCDC42
    亚型
    IgG
  • 应用备注
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    限制
    仅限研究用
  • 生效 #103396 (Immunocytochemistry)
    '独立验证'标志
    by
    Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
    No.
    #103396
    日期
    2018.10.12
    抗原
    CCDC42
    Lot Number
    AH09037940
    Method validated
    Immunocytochemistry
    Positive Control
    NIH/3T3 cells transfected with Ccdc42-mCherry expression vector
    Negative Control
    NIH/3T3 cell lysates
    Notes

    ABIN872760 specifically detects exogenously expressed Ccdc42-Cherry but shows also unspecific background staining.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN872760 
    Secondary Antibody
    goat anti-rabbit 488 (MoBiotec MFP-A1008, lot 2806F058)
    Full Protocol
    • Grow NIH/3T3 cells (ATCC, CRL-1658) in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco 15140-122, lot 1924797), at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with mCherry-tagged Ccdc42 (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
    • Grow cells for 24h.
    • Fix cells in methanol for 10min at -20°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
    • Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at RT.
    • Incubate cells with primary rabbit anti-CCDC42 antibody (antibodies-online, ABIN872760, lot AH09037940) diluted 1:100 in PBS for 1h at 37°C.
    • Wash cells 3x 5min with 1x PBS.
    • Incubate cells with secondary goat anti-rabbit 488 antibody (MoBiotec MFP-A1008, lot 2806F058) diluted 1:300 in PBS for 1h at 37°C.
    • DNA counterstained with DAPI (4’,6-Diamidino-2-phenylindole; Sigma D-9542).
    • Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
    Experimental Notes
  • 生效 #103397 (Western Blotting)
    '独立验证'标志
    by
    Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
    No.
    #103397
    日期
    2018.10.12
    抗原
    CCDC42
    Lot Number
    AH09037940
    Method validated
    Western Blotting
    Positive Control
    Lysate from HEK293 cells transfected with mCherry tagged Ccdc42 expression vector
    Negative Control
    Lysate from untransfected HEK293 cells
    Notes

    Passed. ABIN872760 specifically detects Ccdc42 by immunoblotting.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN872760 
    Secondary Antibody
    goat anti-rabbit IgG (H+L), HRP-conjugated (Jackson Immuno Research, 111-035-003, lot 123450)
    Full Protocol
    • Grow HEK293 (ATCC) cells in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797), at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with mCherry tagged Ccdc42 (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
    • Grow cells for 24h.
    • Lyse cells in SDS-sample buffer and denature total cellular lysates for 5min at 95°C.
    • Separate up to 40µl of protein lysate on a denaturing 10% SDS polyacrylamide gel (Laemmli (1970)).
    • Transfer proteins onto 0.2µm Protran membrane (GE Healthcare, 10600004, A10043108) with a Western blotting system for 1h at 400A (Towbin et al. (1979)).
    • Block the membrane in TBST (50mM Tris-HCl pH7.4, 150mM NaCl, 0.2% Tween 20) containing 5% dry milk (blocking solution) for 1h at RT.
    • Incubate membrane with primary
      • rabbit anti-Ccdc42 antibody (antibodies-online, ABIN872760, lot AH09037940) diluted 1:500 in blocking solution ON at 4°C or
      • rat anti-RFP antibody (antibodies-online, ABIN334653, lot 090428) diluted 1:500 in blocking solution ON at 4°C.
    • Wash membrane for 6x 5min with TBST at RT.
    • Incubate membrane with secondary
      • goat anti-rabbit IgG (H+L), HRP-conjugated antibody (Jackson Immuno Research, 111-035-003, lot 123450) diluted 1:2000 in blocking solution for 45min at RT or
      • rabbit anti-rat IgG-HRPconjugated antibody (Jackson ImmunoResearch Lab. Inc., 312-035-003, lot 34336) diluted 1:2000 in blocking solution for 45min at RT.
    • Wash membranes for 45 min at RT with TBST.
    • Reveal protein bands using Clarity Max Western ECL substrate (Bio-Rad, 1705062) and capture images via Chemidoc Imaging System (Bio-Rad).
    Experimental Notes

    Expected molecular mass of Ccdc42-tagged Cherry is approximately 68kDa. ABIN872760 detects a protein in the expected molecular mass range. The anti-RedFP antibody detects a protein with a similar mass in addition to proteins with lower molecular masses, which are most probably degradation products.

  • 状态
    Liquid
    浓度
    1 μg/μL
    缓冲液
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    有效期
    12 months
  • 抗原
    CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
    别名
    CCDC42 (CCDC42 产品)
    别名
    RGD1565925 antibody, CCDC42A antibody, A530001H01Rik antibody, coiled-coil domain containing 42 antibody, Ccdc42 antibody, CCDC42 antibody
    背景

    Synonyms: CCDC42A, Coiled coil domain containing 42, Coiled coil domain containing protein 42A, FLJ32734, OTTMUSP00000006142, RGD1565925, RP23-400O5.1, CCD42_HUMAN.

    Background: The coiled-coil domain is a structural motif found in proteins that are involved in a diverse array of biological functions such as the regulation of gene expression, cell division, membrane fusion and drug extrusion and delivery. CCDC42 (coiled-coil domain containing 42) is a 316 amino acid protein encoded by a gene that maps to human chromosome 17p13.1. Encoding over 1,200 genes, chromosome 17 comprises over 2.5 % of the human genome. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome.

    基因ID
    146849
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