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DNASE2 抗体 (AA 25-90)

Name: DNASE2 抗体 (AA 25-90)
SKU: ABIN872658
Availability: OutOfStock

DNASE2 适用: 人, 小鼠 ELISA, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse stomach tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-DNase II Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (DNASE2 抗体 (AA 25-90))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-DNase II Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (DNASE2 抗体 (AA 25-90))

IHC (p)

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-DNase II Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (DNASE2 抗体 (AA 25-90))

IHC (p)

Human colon cancer 4% Paraformaldehyde-fixed and paraffin-embedded. (DNASE2 抗体 (AA 25-90))

IF (p)

5 images

(1 reference)

产品编号 ABIN872658

发货至: 中国

Datasheet as PDF

抗原 See all DNASE2 抗体

DNASE2 (Deoxyribonuclease II, Lysosomal (DNASE2))
抗原表位
14

5

4

3

2

1

1

1
AA 25-90
适用
37

20

2
人, 小鼠
宿主
35

3
兔
Compatible Secondaries
克隆类型
38
多克隆
标记
12

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This DNASE2 antibody is un-conjugated
应用范围
14

13

12

12

4

4

2

1

1
ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

人, 小鼠

预测反应

Rat,Dog,Cow,Sheep,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human DNase II alpha

亚型

IgG
anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 19-152) antibody
DNASE2 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 261-360) antibody
DNASE2 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 1-360) antibody
DNASE2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 1-360) antibody
DNASE2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 347-360) antibody
DNASE2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 201-300) antibody
DNASE2 适用: 人, 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 201-300) antibody (Biotin)
DNASE2 适用: 人, 小鼠 ELISA, WB 宿主: 兔 Polyclonal Biotin

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 25-90) antibody (Biotin)
DNASE2 适用: 人, 小鼠 ELISA, IHC (p) 宿主: 兔 Polyclonal Biotin

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 25-90) antibody (AbBy Fluor® 488)
DNASE2 适用: 人, 小鼠 IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 488

anti-Deoxyribonuclease II, Lysosomal (DNASE2) (AA 25-90) antibody (AbBy Fluor® 350)
DNASE2 适用: 人, 小鼠 IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 350

应用备注

ELISA 1:500-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Takahashi, Loo, Okada, Kamachi, Watanabe, Wakita, Watanabe, Kawamoto, Miyata, Barber, Ohtani, Hara: "Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells." in: Nature communications, Vol. 9, Issue 1, pp. 1249, (2018) (PubMed).
抗原

DNASE2 (Deoxyribonuclease II, Lysosomal (DNASE2))

别名

DNase 2 alpha (DNASE2 产品)

背景

Synonyms: DNL, DNL2, DNASE2A, Deoxyribonuclease-2-alpha, Acid DNase, Deoxyribonuclease II alpha, DNase II alpha, Lysosomal DNase II, R31240_2, DNASE2
Background: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.

基因ID

1777

UniProt

O00115