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HA-Tag 抗体

WB, IF, ICC 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN871606
发货至: 中国
  • 抗原 See all HA-Tag products
    HA-Tag
    适用
    请咨询
    宿主
    • 77
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    小鼠
    克隆类型
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    单克隆
    标记
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    This HA-Tag antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    序列
    YPYDVPDYA
    特异性
    HA-Tag mouse mAb detects over-expressed proteins containing the HA epitope tag.
  • 应用备注
    Western blotting 1:5000. Immunofluorescence 1:1000. Immunoprecipitation 1:100. ELISA 1:2000
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    储存条件
    -20 °C
  • 抗原
    HA-Tag
    Abstract
    HA-Tag 产品
    物质类
    Tag
    背景
    This monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the infuenza hemagglutinin epitope (YPYDVPDYA) (KLH-coupled). Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allow for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.
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